CAJ | 학술논문

目的：研究朱槿花苞提取物对小鼠皮肤疖痈溃疡模型的治疗作用，并对其可能作用机制进行探讨。方法将昆明小鼠分为模型组、阳性药组和朱槿花苞正丁醇萃取物( HrBN)组，采用小鼠疖痈模型，观察给药后实验结果。将昆明小鼠分为模型组、阳性药组和HrBN低、中、高剂量组，采用小鼠全层切除模型，观察给药后实验结果。通过体外抗菌实验检测朱槿花苞提取物抗菌MIC和抑菌圈。通过碳粒廓清实验测定朱槿花苞提取物对小鼠巨噬细胞廓清作用的影响，计算 K 和α值。结果 HrBN 对疖痈溃疡修复效果明显，并呈现量效关系。 HrBN、朱槿花苞乙酸乙酯萃取物( HrBE)及AB-8树脂体积分数0．3乙醇段、0．6乙醇段的抗菌作用明显。 HrBN及朱槿花苞乙醇提物( HrBA)高剂量能够增加K和α值。结论 HrBN在动物实验中体现了良好的皮肤疖痈溃疡修复作用，其疗效与抗菌活性和免疫增强作用可能有关。
목적：연구주근화포제취물대소서피부절옹궤양모형적치료작용，병대기가능작용궤제진행탐토。방법장곤명소서분위모형조、양성약조화주근화포정정순췌취물( HrBN)조，채용소서절옹모형，관찰급약후실험결과。장곤명소서분위모형조、양성약조화HrBN저、중、고제량조，채용소서전층절제모형，관찰급약후실험결과。통과체외항균실험검측주근화포제취물항균MIC화억균권。통과탄립곽청실험측정주근화포제취물대소서거서세포곽청작용적영향，계산 K 화α치。결과 HrBN 대절옹궤양수복효과명현，병정현량효관계。 HrBN、주근화포을산을지췌취물( HrBE)급AB-8수지체적분수0．3을순단、0．6을순단적항균작용명현。 HrBN급주근화포을순제물( HrBA)고제량능구증가K화α치。결론 HrBN재동물실험중체현료량호적피부절옹궤양수복작용，기료효여항균활성화면역증강작용가능유관。
Aim To test the skin healing and repairing efficacy and the mechanism of Hibiscus rosa-sinensis L bud extract by using the animal models. Methods KM mice were randomly divided into three groups:the model group, the positive control group, and the n-bu-tyl alcohol extract ( HrBN) group. Using the boils and carbuncles model, the healing condition of all the animals were observed. KM mice were kept in the SPF condition room and divided into five groups: the model group, the positive control group, and the low, middle, high dose groups. Using the full-thickness loss model, the repairing results of all the mice were ob-served. Through the antimicrobial test, the results of MIC and inhibition zone were obtained. The carbon clearance test was used to collect the blood at the time 5min and 15min, and get the liver and spleen, and the results of K andαwere obtained. Results In vivo ex-periments showed there was significant difference be-tween groups;the HrBN extract had the outstanding ef-ficacy in healing and repairing skin boils and full-thickness loss models. It had higher recovery rate than other ethanol extract, such as ethyl acetate extract and chloroform extract. In vitro experiments showed that the HrBN extract, ethyl acetate extract ( HrBE) ,AB-8 macroporous resin 30% alcohol part and 60% alcohol part had obvious antimicrobial efficacy. The carbon clearance test showed HrBN had a good effect in im-proving immune function, and it can increase the K and α. Conclusion HrBN in animal models exerts good skin healing and repairing efficacy, which might be related to its antibacterial activity and immunologic enhancement function.