药学研究
藥學研究
약학연구
JOURNAL OF PHARMACEUTICAL RESEARCH
2015年
8期
447-448,496
,共3页
补肾活血止痛丸%阿魏酸%高效液相色谱法
補腎活血止痛汍%阿魏痠%高效液相色譜法
보신활혈지통환%아위산%고효액상색보법
Bushen Huoxue Zhitong Pills%Ferulic acid%HPLC
目的：建立高效液相色谱法测定补肾活血止痛丸中阿魏酸的含量测定方法。方法用 C18柱为固定相，以乙腈-0.085%磷酸溶液（14：86）为流动相，检测波长为316 nm。结果阿魏酸进样量在0.02049~0.2049μg范围内呈良好的线性关系，r =1（n =7），其平均回收率（n =6）为99.83%。方法的检测限 LOD 为0.3073 ng，定量限 LOQ 为1.0243 ng。结论此方法可靠、准确、专属性强。
目的：建立高效液相色譜法測定補腎活血止痛汍中阿魏痠的含量測定方法。方法用 C18柱為固定相，以乙腈-0.085%燐痠溶液（14：86）為流動相，檢測波長為316 nm。結果阿魏痠進樣量在0.02049~0.2049μg範圍內呈良好的線性關繫，r =1（n =7），其平均迴收率（n =6）為99.83%。方法的檢測限 LOD 為0.3073 ng，定量限 LOQ 為1.0243 ng。結論此方法可靠、準確、專屬性彊。
목적：건립고효액상색보법측정보신활혈지통환중아위산적함량측정방법。방법용 C18주위고정상，이을정-0.085%린산용액（14：86）위류동상，검측파장위316 nm。결과아위산진양량재0.02049~0.2049μg범위내정량호적선성관계，r =1（n =7），기평균회수솔（n =6）위99.83%。방법적검측한 LOD 위0.3073 ng，정량한 LOQ 위1.0243 ng。결론차방법가고、준학、전속성강。
Objective To establish a method for the determination of ferulic acid in Bushen Huoxue Zhitong Pills by HPLC. Methods The C18 column was used,the mobile phase was consisted of acetonitrile -0. 085% phosphoric acid(14:86), and the detection wavelength was 316 nm. Results The standard curves of ferulic acid was linear in range of 0. 020 49 ~0. 204 9 μg,r = 1(n = 7),and the average recovery was 99. 83%(n = 6). The limit of detection was 0. 307 3 ng and the limit of quantification was 1. 024 3 ng. Conclusion The method was reliable,accurate and specific.
