动物医学进展
動物醫學進展
동물의학진전
PROGRESS IN VETERINARY MEDICINE
2015年
8期
114-119
,共6页
奶牛%子宫内膜炎%病原菌%分离鉴定%药敏试验
奶牛%子宮內膜炎%病原菌%分離鑒定%藥敏試驗
내우%자궁내막염%병원균%분리감정%약민시험
dairy cows%endometritis%pathogenic bacterium%isolation and identification%drug sensitivity test
为调查宁夏6个规模化牛场奶牛子宫内膜炎主要致病菌的种类及其耐药情况，采集78头患子宫内膜炎奶牛的子宫分泌物，分离培养细菌，根据各分离菌株的生化反应和16 S rDNA 序列遗传进化分析结果，对致病菌分类鉴定，并对其进行抗生素耐药性分析。结果表明，大肠埃希菌检出率为55．56％，金黄色葡萄球菌检出率为35．90％，其他菌株检出率为8．54％，单一菌株感染患牛占42．31％，混合菌株感染占52．56％。大肠埃希菌和金色葡萄球菌对青霉素 G、氨苄西林、四环素、复方新诺明、链霉素、卡拉霉素耐药性较强（50％～100％），对万古霉素、头孢西丁、头孢噻肟较为敏感（耐药率小于20％）。宁夏6个规模化牛场奶牛子宫内膜炎主要致病菌为大肠埃希菌和金黄色葡萄球菌，且以混合感染为主。该类病原对青霉素类、四环素类、氨基糖甙类和磺胺类抗生素耐药性较强，对万古霉素、头孢类较敏感。
為調查寧夏6箇規模化牛場奶牛子宮內膜炎主要緻病菌的種類及其耐藥情況，採集78頭患子宮內膜炎奶牛的子宮分泌物，分離培養細菌，根據各分離菌株的生化反應和16 S rDNA 序列遺傳進化分析結果，對緻病菌分類鑒定，併對其進行抗生素耐藥性分析。結果錶明，大腸埃希菌檢齣率為55．56％，金黃色葡萄毬菌檢齣率為35．90％，其他菌株檢齣率為8．54％，單一菌株感染患牛佔42．31％，混閤菌株感染佔52．56％。大腸埃希菌和金色葡萄毬菌對青黴素 G、氨芐西林、四環素、複方新諾明、鏈黴素、卡拉黴素耐藥性較彊（50％～100％），對萬古黴素、頭孢西丁、頭孢噻肟較為敏感（耐藥率小于20％）。寧夏6箇規模化牛場奶牛子宮內膜炎主要緻病菌為大腸埃希菌和金黃色葡萄毬菌，且以混閤感染為主。該類病原對青黴素類、四環素類、氨基糖甙類和磺胺類抗生素耐藥性較彊，對萬古黴素、頭孢類較敏感。
위조사저하6개규모화우장내우자궁내막염주요치병균적충류급기내약정황，채집78두환자궁내막염내우적자궁분비물，분리배양세균，근거각분리균주적생화반응화16 S rDNA 서렬유전진화분석결과，대치병균분류감정，병대기진행항생소내약성분석。결과표명，대장애희균검출솔위55．56％，금황색포도구균검출솔위35．90％，기타균주검출솔위8．54％，단일균주감염환우점42．31％，혼합균주감염점52．56％。대장애희균화금색포도구균대청매소 G、안변서림、사배소、복방신낙명、련매소、잡랍매소내약성교강（50％～100％），대만고매소、두포서정、두포새우교위민감（내약솔소우20％）。저하6개규모화우장내우자궁내막염주요치병균위대장애희균화금황색포도구균，차이혼합감염위주。해류병원대청매소류、사배소류、안기당대류화광알류항생소내약성교강，대만고매소、두포류교민감。
To evaluate the species and drug resistance of main pathogenic bacteria causing cow endometri-tis,this experiment was performed to isolate bacteria from the excretion of uteruses of 78 affected cows in six large-scale dairy farms located in Ningxia.The main isolates were classified according to the results of biochemical identification and phylogenetic analysis based on 16 S rDNA sequences.The drug sensitivity tests of the main isolates were conducted.The results indicated that 55.56 percent of pathogens are Esche-richia coli and 35.90 percent of pathogens are Staphylococcus aureus .Other species of pathogens accoun-ted for 8.54 percent of all isolates.42.31 percent of all affected cows infected with single pathogen.It ac-counted for 52.56 percent of affected cows that infected with mixed pathogens.The isolated E.coli and S. aureus were resistant to the majority of antibacterial agents such as penicillin G,ampicillin,tetracycline, chemitrim,streptomycin and kanamycin.However,the E.coli and S.aureus were hypersensitive to van-comycin,cefoxitin and cefotaxime.E.coli and Staphylococcus aureus are main pathogenic bacteria causing endometritis of cows in six large-scale dairy farms located in Ningxia.The mixed infection of several spe-cies of bacteria was frequently determined in uteruses of affected cows.These pathogens were widely re-sistant to penicillins,tetracyclines,aminoglycosides and sulfamines.However,the pathogens were hyper-sensitive to vancomycin and cephalosporins.
