CAJ | 학술논문

为确定诱发中华绒螯蟹肠炎的病原菌，筛选潜在的防控药物，采用传统方法从患肠炎的中华绒螯蟹体内分离到一株病原菌（DD5），通过 API 20E 细菌鉴定系统和16 S rDNA 序列分析对菌株 DD5进行了鉴定，并采用 KB 纸片扩散法对菌株 DD5的抗菌药物敏感性进行了测定。结果表明，菌株 DD5为点状产气单胞菌（GenBank 登录号：KP260655），其16 S rDNA 序列与基因库中气单胞菌属菌株的16 S rDNA 序列有99％～100％的同源性，而且与点状产气单胞菌136c 株（GenBank 登录号：EU488688）的亲缘关系最近。此外，菌株 DD5对四环素、链霉素、卡那霉素、庆大霉素、恩诺沙星、氧氟沙星、左氟沙星、复方新诺明等抗菌药物高度敏感。本研究证实点状产气单胞菌是中华绒螯蟹肠炎的病原菌，恩诺沙星、庆大霉素等常规渔药可作为点状产气单胞菌引起的中华绒螯蟹肠炎的防控用药。
위학정유발중화융오해장염적병원균，사선잠재적방공약물，채용전통방법종환장염적중화융오해체내분리도일주병원균（DD5），통과 API 20E 세균감정계통화16 S rDNA 서렬분석대균주 DD5진행료감정，병채용 KB 지편확산법대균주 DD5적항균약물민감성진행료측정。결과표명，균주 DD5위점상산기단포균（GenBank 등록호：KP260655），기16 S rDNA 서렬여기인고중기단포균속균주적16 S rDNA 서렬유99％～100％적동원성，이차여점상산기단포균136c 주（GenBank 등록호：EU488688）적친연관계최근。차외，균주 DD5대사배소、련매소、잡나매소、경대매소、은낙사성、양불사성、좌불사성、복방신낙명등항균약물고도민감。본연구증실점상산기단포균시중화융오해장염적병원균，은낙사성、경대매소등상규어약가작위점상산기단포균인기적중화융오해장염적방공용약。
In order to confirm the bacterial pathogen of Eriocheir sinensis enteritis and screen potential anti-microbial for its control,a pathogenic strain DD5 was isolated from Eriocheir sinensis with enteritis using traditional methods,identified by API 20E bacterial identification system and 16 S rDNA sequence analy-sis.Its antimicrobial susceptibility was also tested by KB method.The results showed that strain DD5 was an Aeromonas punctata strain (GenBank accession No.KP260655),its 16 S rDNA sequence had a homolo-gy of 99% to 100% with those of Aeromonas strains,and had a closest relationship with A.punctata strain 136c (GenBank accession No:EU488688).In addition,strain DD5 was highly sensitive to tetracy-cline,streptomycin,kanamycin,gentamycin,enrofloxacin,ofloxacin,levofloxacin and compound sulfa-methoxazole.This study confirmed A.punctata as a pathogen of Eriocheir sinensis enteritis,and common fishery antimicrobial such as enrofloxacin and gentamycin may be useful in the control of Eriocheir sinensis enteritis caused by A.punctata.