动物医学进展
動物醫學進展
동물의학진전
PROGRESS IN VETERINARY MEDICINE
2015年
8期
93-96
,共4页
刘建钗%刘彦威%白福娟%刘月
劉建釵%劉彥威%白福娟%劉月
류건차%류언위%백복연%류월
消化道%菌群%检测
消化道%菌群%檢測
소화도%균군%검측
digestive tract%microflora%detection method
消化道菌群对维持消化道机能有重要的调节作用。消化道菌群研究方法可分为两种,即微生物方法(分离培养)和分子生物学方法(基因分析),这两种方法各有利弊,分离培养仍然是基本和广泛使用的方法,但只能够检测生长在培养基上的细菌。对于整个消化道的微生物准确的定量和定性分析还有赖于基因分析方法。因此,这两种方法结合将成为了解消化道细菌的生态系统的关键。论文综述了消化道菌群的种类、分布、作用和研究方法,为消化道菌群研究提供参考。
消化道菌群對維持消化道機能有重要的調節作用。消化道菌群研究方法可分為兩種,即微生物方法(分離培養)和分子生物學方法(基因分析),這兩種方法各有利弊,分離培養仍然是基本和廣汎使用的方法,但隻能夠檢測生長在培養基上的細菌。對于整箇消化道的微生物準確的定量和定性分析還有賴于基因分析方法。因此,這兩種方法結閤將成為瞭解消化道細菌的生態繫統的關鍵。論文綜述瞭消化道菌群的種類、分佈、作用和研究方法,為消化道菌群研究提供參攷。
소화도균군대유지소화도궤능유중요적조절작용。소화도균군연구방법가분위량충,즉미생물방법(분리배양)화분자생물학방법(기인분석),저량충방법각유리폐,분리배양잉연시기본화엄범사용적방법,단지능구검측생장재배양기상적세균。대우정개소화도적미생물준학적정량화정성분석환유뢰우기인분석방법。인차,저량충방법결합장성위료해소화도세균적생태계통적관건。논문종술료소화도균군적충류、분포、작용화연구방법,위소화도균군연구제공삼고。
Microflora in the digestive tract is an important regulatory role in maintaining the function of di-gestive system.These methods of detection are generally divided into two method-microbiological method (traditional culture method)and molecular biology technique(genetic analysis).Both techniques have ad-vantages and disadvantages.The microbiological technique is only able to detect identifiable bacteria that can be grown on available substrates.Nevertheless,the culture is still a fundamental and widely used method for detection of digestive tract microorganisms.However,for an accurate quantitative and qualita-tive investigation of the microbiology of the entire gastrointestinal tract,genetic method is required.The combination of both methods may become a critical element for understanding the ecosystem of digestive tract bacteria.This paper reviewed the functions,kinds,distribution and methods of detection in digestive tract microorganism to establish the basis for gastrointestinal tract microflora.