CAJ | 학술논문

为了对芽孢杆菌发酵产β-葡聚糖酶的培养基进行进一步的研究，以芽孢杆菌为发酵菌，通过单因素试验和三因素三水平正交试验法对芽孢杆菌的10 L发酵罐发酵产酶培养基进行优化。实验结果表明：芽孢杆菌产β-葡聚糖酶的最优培养基为甘油含量6 g／L、酵母粉含量24 g／L 和NaCl 含量10 g／L；发酵条件：接种量为10％、初始发酵pH 值为7、培养温度为37℃、转速为350～950 r／min、通风量为1 vvm和发酵时间15 h。经过3批发酵实验验证，最优条件下β-葡聚糖酶最高酶活可达3112 U／mL。
위료대아포간균발효산β-포취당매적배양기진행진일보적연구，이아포간균위발효균，통과단인소시험화삼인소삼수평정교시험법대아포간균적10 L발효관발효산매배양기진행우화。실험결과표명：아포간균산β-포취당매적최우배양기위감유함량6 g／L、효모분함량24 g／L 화NaCl 함량10 g／L；발효조건：접충량위10％、초시발효pH 치위7、배양온도위37℃、전속위350～950 r／min、통풍량위1 vvm화발효시간15 h。경과3비발효실험험증，최우조건하β-포취당매최고매활가체3112 U／mL。
Focus on the medium optimization of the beta glucan enzyme fermentation.With single-factor experiment and three factors and three levels orthogonal experiment,the culture medium for bacillus (10 L fermentation tank)enzyme fermentation is optimized.The final experiment result indicates the optimal medium conditions for the beta glucan enzyme fermentation are as follows:glycerol of 6 g/L,yeast powder of 24 g/L,NaCl of 10 g/L,inoculation dose of 10%,initial fermentation pH of 7,culture temperature of 37 ℃,rotating speed of 350~950 r/min,ventilation rate of 1 vvm and fermentation time of 15 h.A series of three fermentation experiments point out that the highest enzyme activity can reach 3112 U/mL under the optimal conditions.