CAJ | 학술논문

目的：观察牛磺酸、丹血通（丹参联合血栓通）和灯盏花素对青春前期大鼠睾丸扭转复位后健侧睾丸的远期影响及其保护作用，比较3种药物对抗睾丸缺血再灌注损伤的保护作用。方法：64只4周龄健康雄性SD大鼠，随机分为假手术组、模型组、牛磺酸单次和连续给药组、丹血通单次和连续给药组以及灯盏花素单次和连续给药组，每组8只，建立左侧睾丸扭转复位动物模型（720°，2 h）。于术后6周处死大鼠，取右侧睾丸及附睾，检测睾丸组织总抗氧化能力（ T－AOC）、超氧化物歧化酶（ SOD）活性、一氧化氮合酶（ NOS）活性和丙二醛（ MDA）含量，测定精子畸形率和精子活动率和精子浓度，行睾丸组织病理学观察。结果：与模型组相比，3个连续组的SOD活性、T－AOC、精子活动率和精子浓度均增加，MDA含量和精子畸形率均降低（ P＜0．05）。3个单次组的SOD活性和精子活动率（除灯盏花素单次组）均增加，MDA含量和精子畸形率均降低，牛磺酸单次组T－AOC和精子浓度增加（ P＜0．05）。模型组可见生精小管退变，间质水肿，其它给药组睾丸扭转复位诱发的组织学改变明显改善。结论：青春前期大鼠单侧睾丸扭转复位后可致健侧睾丸缺血再灌注损伤，牛磺酸、丹血通和灯盏花素均对青春前期大鼠睾丸扭转复位后健侧睾丸远期功能恢复起到一定的保护作用，其作用效果牛磺酸明显优于丹血通和灯盏花素，丹血通明显优于灯盏花素，且连续给药明显优于单次给药。
목적：관찰우광산、단혈통（단삼연합혈전통）화등잔화소대청춘전기대서고환뉴전복위후건측고환적원기영향급기보호작용，비교3충약물대항고환결혈재관주손상적보호작용。방법：64지4주령건강웅성SD대서，수궤분위가수술조、모형조、우광산단차화련속급약조、단혈통단차화련속급약조이급등잔화소단차화련속급약조，매조8지，건립좌측고환뉴전복위동물모형（720°，2 h）。우술후6주처사대서，취우측고환급부고，검측고환조직총항양화능력（ T－AOC）、초양화물기화매（ SOD）활성、일양화담합매（ NOS）활성화병이철（ MDA）함량，측정정자기형솔화정자활동솔화정자농도，행고환조직병이학관찰。결과：여모형조상비，3개련속조적SOD활성、T－AOC、정자활동솔화정자농도균증가，MDA함량화정자기형솔균강저（ P＜0．05）。3개단차조적SOD활성화정자활동솔（제등잔화소단차조）균증가，MDA함량화정자기형솔균강저，우광산단차조T－AOC화정자농도증가（ P＜0．05）。모형조가견생정소관퇴변，간질수종，기타급약조고환뉴전복위유발적조직학개변명현개선。결론：청춘전기대서단측고환뉴전복위후가치건측고환결혈재관주손상，우광산、단혈통화등잔화소균대청춘전기대서고환뉴전복위후건측고환원기공능회복기도일정적보호작용，기작용효과우광산명현우우단혈통화등잔화소，단혈통명현우우등잔화소，차련속급약명현우우단차급약。
[ABSTRACT]AIM:Toobservethelong-termeffectsoftaurine,Danxuetong(Salviamiltiorrhizacombinedwith Xueshuantong ) and breviscapine on contralateral testis with unilateral testicular torsion in prepubertal rats , and to compare their protective effect on testis with ischemia reperfusion injury .METHODS: Four-week-old healthy male SD rats ( n=64) were randomly divided into 8 groups:sham operation group, saline group, single-dose and successive taurine groups, single-dose and successive Danxuetong groups , and single-dose and successive breviscapine groups .The animal model of left testicular torsion reduction (720°for 2 h) was established.After 6 weeks of surgery, the right testes and epididymides from the rats were collected immediately to detect the total antioxidant capacity (T-AOC), the activity of superoxide dis-mutase (SOD), the activity of nitric oxide synthase (NOS) and the content of malondialdehyde (MDA).The sperm de-formity, sperm viability and sperm concentration were measured , and the pathological examination of the testicular tissues was performed.RESULTS:Compared with saline group, the activity of SOD, T-AOC, sperm viability and sperm concen-tration were increased , while MDA content and sperm deformity were decreased in 3 successive groups .SOD activity and sperm viability ( except single-dose breviscapine group ) were increased , but MDA content and sperm deformity were de-creased in 3 single-dose groups .T-AOC activity and sperm concentration were increased in single-dose taurine group .The saline group showed interstitial edema and degeneration of seminiferous tubules , and the rest groups showed a significant improvement in histological morphology .CONCLUSION:The contralateral testis in prepubertal rats was damaged after u-nilateral testicular torsion , and taurine , Danxuetong and breviscapine have long-term protective effect on the tissues under ischemia-reperfusion injury .The protective effect of Danxuetong is better than breviscapine , and taurine is the best of them.The effect of single-dose group is better than the successive application .