中国病理生理杂志
中國病理生理雜誌
중국병리생리잡지
CHINESE JOURNAL OF PATHOPHYSIOLOGY
2015年
8期
1432-1436
,共5页
巢蛋白%肿瘤侵袭%细胞迁移%食管癌
巢蛋白%腫瘤侵襲%細胞遷移%食管癌
소단백%종류침습%세포천이%식관암
Nestin%Neoplasminvasion%Cellmigration%Esophageal
目的：探讨巢蛋白（nestin）对食管鳞癌ECA109细胞的侵袭与迁移能力的影响及可能的分子机制。方法：通过靶向沉默食管癌ECA109细胞内源性nestin基因后，采用real－time PCR和Western blot分别检测nestin基因的干扰效率及细胞内MMP2、MMP9、VEGF及β－catenin的表达水平；Transwell实验检测细胞侵袭和迁移能力的变化。结果：与control－siRNA组相比， nestin－siRNA组中nestin mRNA和蛋白水平明显降低；细胞侵袭迁移能力受到明显抑制；MMP2，MMP9及VEGF表达明显降低；细胞总β－catenin及核内β－catenin表达明显减少。结论：Nestin与食管癌侵袭迁移能力密切相关，下调nestin表达可明显抑制食管癌细胞ECA109的侵袭迁移能力，其机制可能与抑制β－catenin的核内转移与下调MMP2、MMP9及VEGF的表达有关。
目的：探討巢蛋白（nestin）對食管鱗癌ECA109細胞的侵襲與遷移能力的影響及可能的分子機製。方法：通過靶嚮沉默食管癌ECA109細胞內源性nestin基因後，採用real－time PCR和Western blot分彆檢測nestin基因的榦擾效率及細胞內MMP2、MMP9、VEGF及β－catenin的錶達水平；Transwell實驗檢測細胞侵襲和遷移能力的變化。結果：與control－siRNA組相比， nestin－siRNA組中nestin mRNA和蛋白水平明顯降低；細胞侵襲遷移能力受到明顯抑製；MMP2，MMP9及VEGF錶達明顯降低；細胞總β－catenin及覈內β－catenin錶達明顯減少。結論：Nestin與食管癌侵襲遷移能力密切相關，下調nestin錶達可明顯抑製食管癌細胞ECA109的侵襲遷移能力，其機製可能與抑製β－catenin的覈內轉移與下調MMP2、MMP9及VEGF的錶達有關。
목적：탐토소단백（nestin）대식관린암ECA109세포적침습여천이능력적영향급가능적분자궤제。방법：통과파향침묵식관암ECA109세포내원성nestin기인후，채용real－time PCR화Western blot분별검측nestin기인적간우효솔급세포내MMP2、MMP9、VEGF급β－catenin적표체수평；Transwell실험검측세포침습화천이능력적변화。결과：여control－siRNA조상비， nestin－siRNA조중nestin mRNA화단백수평명현강저；세포침습천이능력수도명현억제；MMP2，MMP9급VEGF표체명현강저；세포총β－catenin급핵내β－catenin표체명현감소。결론：Nestin여식관암침습천이능력밀절상관，하조nestin표체가명현억제식관암세포ECA109적침습천이능력，기궤제가능여억제β－catenin적핵내전이여하조MMP2、MMP9급VEGF적표체유관。
AIM:ToinvestigatetheeffectofsmallinterferenceRNA(siRNA)-mediatedsilencingofnestin gene on the invasion and migration of human esophageal cancer ECA 109 cells and the possible mechanism .METHODS:The esophageal cell line ECA 109 was transfected with siRNA targeting nestin and the cell invasion and migration abilities were observed.The expression of nestin, MMP2, MMP9, VEGF, and total and nuclear β-catenin proteins in the transfec-ted cells were determined by real-time PCR and Western blot .RESULTS:Compared with control group , the expression of nestin at mRNA and protein levels was significantly down-regulated in the ECA109 cells transfected with nestin-siRNA, so was the expression of MMP2, MMP9, VEGF, and total and nuclear β-catenin proteins.The levels of invasion and migra-tion capacities of ECA 109 cells transfected with nestin-siRNA were lower than those in the cells transfected with control-siRNA.CONCLUSION:Knockdown of nestin expression significantly inhibits the invasion and migration of the esophage-al cancer cells , which may act via suppressing β-catenin translocation to the nucleus and influencing the expression of MMP2, MMP9 and VEGF.
