CAJ | 학술논문

目的：通过检测P21活化激酶1（PAK1）和淋巴样增强结合因子1（LEF1）在食道癌组织中的表达以及对食道癌细胞株增殖的影响，探讨PAK1和LEF1在食道癌增殖中的作用。方法：采用实时荧光定量PCR方法检测食道癌组织中PAK1和LEF1 mRNA的表达，通过基因克隆构建PAK1和LEF1质粒，转染食道癌细胞株KYSE，MTT法检测细胞活力，流式细胞术检测细胞凋亡。结果：PAK1在食道癌组织中表达降低，LEF1在食道癌组织中表达增高，PAK1的表达与LEF1的表达呈负性相关；单独转染PAK1和LEF1能够增加食道癌细胞株KYSE的增殖，PAK1和LEF1共同转染的增殖率反而不及单独各自转染的增殖率。 PAK1和LEF1单独或共同转染均不影响KYSE细胞凋亡。结论：在食道癌组织中，PAK1低表达，LEF1和转录因子4（ TCF4）高表达；LEF1在食道癌细胞增殖中起主要作用，提示LEF1可能成为诊断食道癌的生物标记物和食道癌治疗的靶点。
목적：통과검측P21활화격매1（PAK1）화림파양증강결합인자1（LEF1）재식도암조직중적표체이급대식도암세포주증식적영향，탐토PAK1화LEF1재식도암증식중적작용。방법：채용실시형광정량PCR방법검측식도암조직중PAK1화LEF1 mRNA적표체，통과기인극륭구건PAK1화LEF1질립，전염식도암세포주KYSE，MTT법검측세포활력，류식세포술검측세포조망。결과：PAK1재식도암조직중표체강저，LEF1재식도암조직중표체증고，PAK1적표체여LEF1적표체정부성상관；단독전염PAK1화LEF1능구증가식도암세포주KYSE적증식，PAK1화LEF1공동전염적증식솔반이불급단독각자전염적증식솔。 PAK1화LEF1단독혹공동전염균불영향KYSE세포조망。결론：재식도암조직중，PAK1저표체，LEF1화전록인자4（ TCF4）고표체；LEF1재식도암세포증식중기주요작용，제시LEF1가능성위진단식도암적생물표기물화식도암치료적파점。
AIM:ToinvestigatetheeffectofP21-activatedkinase1(PAK1)andlymphoidenhancer-binding factor 1(LEF1) on the proliferation of esophagus cancer cells .METHODS:Real-time PCR was applied to detect the mR-NA expression of PAK1 and LEF1 in the esophagus cancer tissues .MTT assay were used to measure the proliferation of hu-man esophagus cancer cell line KYSE transfected with PAK 1 and LEF1.RESULTS: The mRNA expression of PAK1 in the esophagus cancer tissues was lower than that in control group (P<0.05).The mRNA expression of LEF1 and tran-scription factor 4 (TCF4) in the esophagus cancer tissues was higher than that in control group (P<0.05).The prolifera-tion of KYSE cells with over-expression of PAK1 and LEF1 was higher than that in control group .No significant change of apoptosis between the KYSE cells with over-expression of PAK1 and LEF1 and control group was observed .CONCLU-SION:The expression of PAK1 decreases and the expression of LEF 1 increases in esophagus cancer tissues .LEF1 domi-nantly regulates the proliferation of esophagus cancer cells .