CAJ | 학술논문

目的：研究白酱感冒颗粒的提取工艺及其质量标准。方法：选用L9（34）进行正交设计实验，对白酱感冒颗粒的提取工艺进行筛选，以东莨菪内酯含量为指标，考察提取溶剂、提取时间、热回流速度、溶剂倍数4个因素；采用HPLC法测定东莨菪内酯的含量，色谱柱为依利特C18色谱柱（4．6mm ×250mm，5μm）；流动相为乙腈－水－冰醋酸（15∶85∶0．4）；检测波长为343nm。结果：采用热回流提取技术加水煎煮2次，第一次提取2h，第二次提取1h，每次加水15倍，热回流速度为1．5L／h（以每kg药材计）提取效果最好；东莨菪内酯在2～12μg／mL线性关系良好（r＝0．9998），平均回收率100．5％，RSD为1．69％。结论：该方法稳定可靠，适合工业化大生产。
목적：연구백장감모과립적제취공예급기질량표준。방법：선용L9（34）진행정교설계실험，대백장감모과립적제취공예진행사선，이동랑탕내지함량위지표，고찰제취용제、제취시간、열회류속도、용제배수4개인소；채용HPLC법측정동랑탕내지적함량，색보주위의리특C18색보주（4．6mm ×250mm，5μm）；류동상위을정－수－빙작산（15∶85∶0．4）；검측파장위343nm。결과：채용열회류제취기술가수전자2차，제일차제취2h，제이차제취1h，매차가수15배，열회류속도위1．5L／h（이매kg약재계）제취효과최호；동랑탕내지재2～12μg／mL선성관계량호（r＝0．9998），평균회수솔100．5％，RSD위1．69％。결론：해방법은정가고，괄합공업화대생산。
Objective:To study the extraction process and quality standard of the Baijiang Ganmao Granule.Methods: Making an L9 (34 ) orthogonal experimental design, with the content of Scopoletin as the evaluation index and with the extraction solvent ,extraction time, heat reflux rate, solvent multiples as the four factors.Finding out a optimization of the Baijiang Ganmao Granule extraction process;Using HPLC determination of Scopoletin, the column was Elite C18 column (4.6mm ×250mm, 5μm);mobile phase of ace-tonitrile -water -acetic acid (15∶85∶0.4); detection wavelength of 343nm.Results: Using the heat reflux extraction technology boil water 2 times, the first extract 2 hours and the second 1 hour, added 15 times of water every time, Heat reflux rate 1.5L/h ( per kg herbs) extracts the best;good linear indications showed as a results of the scopoletin being controlled within 2~12μg/mL ( r=0.9998), the average recovery was 100.5%, RSD is 1.69%.Conclusion:The method was stable and reliable, suitable for industrial production.