CAJ | 학술논문

目的：观察骨髓间充质干细胞膜微粒（MSC－MPs）对大鼠心肌梗死后血管新生以及心功能的影响。方法：提取Sprague－Dawley大鼠MSCs并培养，在低氧低营养条件下培养72 h，以诱导细胞凋亡释放MSC－MPs。将培养上清液超速离心获取MSC－MPs，透射电镜下观察其大小及形态，并用流式细胞术分析其表型。建立SD大鼠心肌梗死模型，心肌梗死边缘区注射膜微粒及对照试剂。超声心动图检测心功能，Masson染色检测心梗面积，免疫组织化学染色技术检测梗死边缘区血管α－平滑肌肌动蛋白和von Willebrand因子以确定血管新生情况，real－time PCR检测心梗组织中血管内皮生长因子（VEGF）表达变化。结果：MSCs凋亡后可以释放膜微粒，MSC－MPs来自MSCs，直径为100～1000 nm。心肌梗死大鼠心肌内注射MSC－MPs后，第7天和第28天时心功能明显改善，第28 d 时心梗面积比对照组减小，新生血管密度明显增加，第7天时心梗组织VEGF的表达增加。结论：MSC－MPs可以促进大鼠心肌梗死后的血管新生，改善心功能。
목적：관찰골수간충질간세포막미립（MSC－MPs）대대서심기경사후혈관신생이급심공능적영향。방법：제취Sprague－Dawley대서MSCs병배양，재저양저영양조건하배양72 h，이유도세포조망석방MSC－MPs。장배양상청액초속리심획취MSC－MPs，투사전경하관찰기대소급형태，병용류식세포술분석기표형。건립SD대서심기경사모형，심기경사변연구주사막미립급대조시제。초성심동도검측심공능，Masson염색검측심경면적，면역조직화학염색기술검측경사변연구혈관α－평활기기동단백화von Willebrand인자이학정혈관신생정황，real－time PCR검측심경조직중혈관내피생장인자（VEGF）표체변화。결과：MSCs조망후가이석방막미립，MSC－MPs래자MSCs，직경위100～1000 nm。심기경사대서심기내주사MSC－MPs후，제7천화제28천시심공능명현개선，제28 d 시심경면적비대조조감소，신생혈관밀도명현증가，제7천시심경조직VEGF적표체증가。결론：MSC－MPs가이촉진대서심기경사후적혈관신생，개선심공능。
AIM:Toobservetheeffectsofmicroparticlesderivedfrombonemarrowmesenchymalstemcells ( MSC-MPs) on angiogenesis and cardiac function in a rat myocardial infarction model .METHODS:MSCs were obtained from Sprague-Dawley rats.MSCs were treated under serum-free condition in hypoxia for 72 h, and the microparticles were isolated from the supernatants .The phenotypic profile of MSC-MPs was determined by bead-based flow cytometry and the morphology was observed under a transmission electron microscope .The rat myocardial infarction model was established . The cardiac function was evaluated by echocardiography after the intramyocardial injection of MSC -MPs.The myocardial in-farct size was observed by Masson staining .The blood vessel density in the peri-infarcted area was measured using immuno-histochemical staining for von Willebrand factor and α-smooth muscle actin.The expression of vascular endothelial growth factor ( VEGF) was analyzed by real-time PCR.RESULTS: Apoptotic MSCs released a large quantity of microparticles which were phenotypically similar to the parent MSCs and 100~1 000 nm in diameter.The cardiac functions of myocardial infarction rat model were improved at 7 d and 28 d after intramyocardial injection of MSC-MPs compared with control group . The myocardial infarct size was reduced and angiogenesis was promoted significantly in the infarcted heart injected with MSC-MPs 28 d after treatment .MSC-MPs treatment also increased the expression level of VEGF within 7 d.CONCLU-SION:MSC-MPs protect cardiac tissue from ischemic injury and improve cardiac function by promoting angiogenesis after myocardial infarction .