CAJ | 학술논문

目的：观察钩藤碱对自发性高血压大鼠血压、心肌肥厚及心肌纤维化的影响并探讨其可能的作用机制。方法：32只自发性高血压大鼠随机分为模型组、钩藤碱高（10 mg? kg －1? d－1）、低（2．5 mg? kg－1? d－1）剂量组、卡托普利组（17．5 mg? kg－1? d－1），每组8只。另设8只Wistar－Kyoto大鼠作为正常对照组。分别于给药前和给药后每2周测量尾动脉收缩压（ SBP ）。治疗10周后处死大鼠，取其心脏计算全心重量指数和左心室重量指数；检测心肌中羟脯氨酸（ HYP）及血浆中血管紧张素Ⅱ（ AngⅡ）的含量；HE染色观察心肌病理学变化，Masson染色观察心肌胶原纤维变化；免疫组化法和Western blot法测心肌组织转化生长因子－β1（ TGF－β1）和Smad3蛋白的表达。结果：与模型组相比，钩藤碱能明显降低自发性高血压大鼠的血压（ P＜0．05），降低心肌HYP含量及血浆AngⅡ的含量（P＜0．05），减轻心肌组织的病理损伤和胶原纤维沉积，下调TGF－β1和Smad3蛋白的表达（P＜0．01）。结论：钩藤碱能降低自发性高血压大鼠的血压、调节改善自发性高血压大鼠的心室重构，其机制可能与其影响TGF－β1／Smad通路以及降低AngⅡ含量有关。
목적：관찰구등감대자발성고혈압대서혈압、심기비후급심기섬유화적영향병탐토기가능적작용궤제。방법：32지자발성고혈압대서수궤분위모형조、구등감고（10 mg? kg －1? d－1）、저（2．5 mg? kg－1? d－1）제량조、잡탁보리조（17．5 mg? kg－1? d－1），매조8지。령설8지Wistar－Kyoto대서작위정상대조조。분별우급약전화급약후매2주측량미동맥수축압（ SBP ）。치료10주후처사대서，취기심장계산전심중량지수화좌심실중량지수；검측심기중간포안산（ HYP）급혈장중혈관긴장소Ⅱ（ AngⅡ）적함량；HE염색관찰심기병이학변화，Masson염색관찰심기효원섬유변화；면역조화법화Western blot법측심기조직전화생장인자－β1（ TGF－β1）화Smad3단백적표체。결과：여모형조상비，구등감능명현강저자발성고혈압대서적혈압（ P＜0．05），강저심기HYP함량급혈장AngⅡ적함량（P＜0．05），감경심기조직적병리손상화효원섬유침적，하조TGF－β1화Smad3단백적표체（P＜0．01）。결론：구등감능강저자발성고혈압대서적혈압、조절개선자발성고혈압대서적심실중구，기궤제가능여기영향TGF－β1／Smad통로이급강저AngⅡ함량유관。
AIM:Toinvestigatetheeffectofrhynchophylline(Rhy)onbloodpressure,cardiachypertrophy and myocardial fibrosis in spontaneously hypertensive rats ( SHR) .METHODS:Spontaneously hypertensive rats were ran-domly divided into model group , high dose (10 mg? kg-1? d-1 ) and low dose (2.5 mg? kg-1? d-1 ) group of rhyncho-phylline, captopril group (17.5 mg? kg -1? d-1).Wistar-Kyoto rats were used as normal control.Respectively, systolic blood pressure was measured by tail cuff every 2 weeks.After 10 weeks, heart weight index and left ventricular weight in-dex were calculated .The myocardial hydroxyproline and plasma angiotensin Ⅱwere detected .Moreover , basic myocardial histopathological changes and myocardial collagen fibres were observed by HE staining and Masson staining , respectively . The protein expression of TGF-β1 and Smad3 in the myocardium was measured by the methods of immunohistochemistry and Western blot .RESULTS:Compared with SHR model group , Rhy significantly reduced blood pressure ( P<0.05 ) , the levels of HYP in the myocardium (P<0.05) and the levels of AngⅡin the plasma (P<0.01).The pathological dama-ges of the myocardial tissues and collagen deposition were attenuated .The protein expression of TGF-β1 and Smad3 was sig-nificantly reduced by the treatment with Rhy (P<0.01).CONCLUSION:Rhynchophylline reduces blood pressure and adjusts to improve ventricular remodeling of SHR .The mechanism may be involved in the TGF-β1/Smad pathway and re-ducing AngⅡcontent.