国际移植与血液净化杂志
國際移植與血液淨化雜誌
국제이식여혈액정화잡지
INTERNATIONAL JOURNAL OF TRANSPLANTATION AND HEMOPURIFICATION
2015年
4期
32-35
,共4页
刘涛%王应宝%马超龙%范振磊%李益飞%况应敏
劉濤%王應寶%馬超龍%範振磊%李益飛%況應敏
류도%왕응보%마초룡%범진뢰%리익비%황응민
肾移植%感染%CD64
腎移植%感染%CD64
신이식%감염%CD64
Kidney transplantation%Infection%CD64%Flow cytometry
目的 通过研究肾移植人群感染期以及功能稳定时期外周血中性粒细胞CD64的表达变化,为CD64作为肾移植术后感染的诊断指标提供依据.方法 对2011年2月至2014年5月期间在我院就诊的肾移植受者、血液透析患者共60例进行分组,分为血液透析组、肾移植术后非感染组、肾移植术后感染组,肾移植术后感染痊愈组使用流式细胞术进行中性粒细胞CD64的测定,全自动血细胞分析仪进行外周血细胞分析的测定,并对各组进行统计学分析.结果 透析组、肾移植术后非感染组中性粒细胞和单核细胞上CD64的平均荧光强度分别是145.22±28.95,910.27±337.26;82.06±27.50,550.26±198.36.肾移植术后感染组和肾移植术后感染治愈组中性粒细胞和单核细胞上CD64的平均荧光强度分别是315.29±220.85、1356.27±521.22vs125.19±150.23、725.73±386.92.肾移植术后非感染组与肾移植术后感染组与差异有统计学意义(P＜0.05).结论 肾移植术后感染患者中性粒细胞上的CD64的表达增高,能够作为判断在肾移植术后患者是否感染的指标,具有较高的敏感性及准确性.
目的 通過研究腎移植人群感染期以及功能穩定時期外週血中性粒細胞CD64的錶達變化,為CD64作為腎移植術後感染的診斷指標提供依據.方法 對2011年2月至2014年5月期間在我院就診的腎移植受者、血液透析患者共60例進行分組,分為血液透析組、腎移植術後非感染組、腎移植術後感染組,腎移植術後感染痊愈組使用流式細胞術進行中性粒細胞CD64的測定,全自動血細胞分析儀進行外週血細胞分析的測定,併對各組進行統計學分析.結果 透析組、腎移植術後非感染組中性粒細胞和單覈細胞上CD64的平均熒光彊度分彆是145.22±28.95,910.27±337.26;82.06±27.50,550.26±198.36.腎移植術後感染組和腎移植術後感染治愈組中性粒細胞和單覈細胞上CD64的平均熒光彊度分彆是315.29±220.85、1356.27±521.22vs125.19±150.23、725.73±386.92.腎移植術後非感染組與腎移植術後感染組與差異有統計學意義(P＜0.05).結論 腎移植術後感染患者中性粒細胞上的CD64的錶達增高,能夠作為判斷在腎移植術後患者是否感染的指標,具有較高的敏感性及準確性.
목적 통과연구신이식인군감염기이급공능은정시기외주혈중성립세포CD64적표체변화,위CD64작위신이식술후감염적진단지표제공의거.방법 대2011년2월지2014년5월기간재아원취진적신이식수자、혈액투석환자공60례진행분조,분위혈액투석조、신이식술후비감염조、신이식술후감염조,신이식술후감염전유조사용류식세포술진행중성립세포CD64적측정,전자동혈세포분석의진행외주혈세포분석적측정,병대각조진행통계학분석.결과 투석조、신이식술후비감염조중성립세포화단핵세포상CD64적평균형광강도분별시145.22±28.95,910.27±337.26;82.06±27.50,550.26±198.36.신이식술후감염조화신이식술후감염치유조중성립세포화단핵세포상CD64적평균형광강도분별시315.29±220.85、1356.27±521.22vs125.19±150.23、725.73±386.92.신이식술후비감염조여신이식술후감염조여차이유통계학의의(P＜0.05).결론 신이식술후감염환자중성립세포상적CD64적표체증고,능구작위판단재신이식술후환자시부감염적지표,구유교고적민감성급준학성.
Objective The article attempts to analyze the granulocytes surface expression of CD64 in the group of kidney transplantation through the application of statistical methods,compare mainly with the expression of CD64 of the patients on dialysis,the health kidney post-transplantation,the patients with infection and treatment of renal post-transplantation.In order to evaluate the possibility of CD64 in renal transplantation as an infection of monitoring indicators and provide the clinical evidence for treatment.Methods During the period February 2011 to May 2014,60 cases of renal transplant patients have been done the determination of CD64 on admission,and determination the blood cell analysis from the cubital vein.The group is divided into the following groups based on clinical and blood cell analysis:dialysis group,the normal of renal post-transplantation,renal post-transplantation with infection,the treatment of renal posttransplant.The use of statistical methods:Firstly,analysis of CD64 expression in the dialysis,the normal of post.Secondly,analysis of CD64 expression in the infection and treatment group.The last,analysis of the correlation between the granulocyte CD64 and the percentage of neutrophils.Results The expression levels of neutrophil and monocyte of the mean fluorescence of CD64 in dialysis,normal of post-transplant is145.22 ± 28.95,910.27 ± 337.26;82.06 ± 27.50,550.26 ± 198.36.The expression levels of neutrophil and monocyte of the mean fluorescence of CD64 in the infection and treatment is 315.29 ± 220.85,1356.27 ± 521.22;125.19 ± 150.23,725.73± 386.92.The level of neutrophil and monocyte CD64 in infection is significantly higher than the treatment of infection,(P ＜ 0.05).Conclusion The average fluorescence intensity of CD64 in normal of post-transplant's monocyte is lower than the normal contral and the dialysis.CD64 expression on neutrophils and monocytes will be increased in the renal post-transplantation with infection.Therefore,the expression of CD64 on neutrophils and monocles can be used as an infection of the indicators used in kidney transplant population.
