CAJ | 학술논문

目的：探讨长链非编码 RNA HOTAIR 在胃癌及癌旁组织中的表达及其与临床病理特征之间的关系。方法：利用实时定量 PCR 法检测55例胃癌组织及其配对癌旁正常组织中长链非编码 RNA HOTAIR 的表达情况，并分析其与胃癌临床病理学特征之间的联系。结果：实时定量 PCR 法检测 HOTAIR 在胃癌组织中的相对表达量为0．0232±0．0073，其配对癌旁正常组织相对表达量为0．0071±0．0017，差异具有统计学意义（P ＜0．05）。长链非编码 RNA HOTAIR 的表达与胃癌患者肿瘤淋巴结转移、远处转移、TNM分期显著相关（P＜0．05），与年龄、性别、分化程度等无关（P ＞0．05）。结论：长链非编码 RNA HOTAIR 在胃癌组织中表达显著高于癌旁组织，与胃癌转移、TNM分期等病理因素相关，可能在胃癌的侵袭、转移过程中起重要作用。
목적：탐토장련비편마 RNA HOTAIR 재위암급암방조직중적표체급기여림상병리특정지간적관계。방법：이용실시정량 PCR 법검측55례위암조직급기배대암방정상조직중장련비편마 RNA HOTAIR 적표체정황，병분석기여위암림상병이학특정지간적련계。결과：실시정량 PCR 법검측 HOTAIR 재위암조직중적상대표체량위0．0232±0．0073，기배대암방정상조직상대표체량위0．0071±0．0017，차이구유통계학의의（P ＜0．05）。장련비편마 RNA HOTAIR 적표체여위암환자종류림파결전이、원처전이、TNM분기현저상관（P＜0．05），여년령、성별、분화정도등무관（P ＞0．05）。결론：장련비편마 RNA HOTAIR 재위암조직중표체현저고우암방조직，여위암전이、TNM분기등병리인소상관，가능재위암적침습、전이과정중기중요작용。
Objective:To investigate the relative expression of long non -coding RNA HOTAIR(lncRNA HO-TAIR)in the tissues of gastric cancer,and its correlation with clinicpathological features.Methods:The relative ex-pression of lncRNA HOTAIR in 55 gastric cancer specimens and their corresponding cutting normal specimens was detected by real -time PCR.The relationship between the expression of lncRNA HOTAIR and clinicopathologic char-acteristics was analyzed by SPSS 16.0.Results:The relative expression of lncRNA HOTAIR in gastric cancer tissues was significantly higher than that in their corresponding normal tissues,and it also showed significant correlation with tumor lymph node invasion,distant metastasis and TNMstage(P <0.05),but not related to age,gender,tumor size and differentiation of tumor(P >0.05).Conclusion:High expression of lncRNA HOTAIR in gastric cancer could be associated with the invasion and metastasis of gastric cancer,and HOTAIR probably plays an important role in inva-sion and metastasis in gastric cancer.