CAJ | 학술논문

目的：研究 mTOR 信号通路与非小细胞肺癌放疗敏感性的关系。方法：采用不同剂量的雷帕霉素抑制非小细胞肺癌细胞系 A549中的 mTOR 信号通路；Western blot 检测雷帕霉素作用后下游信号分子的表达；将 A549细胞分为空白对照组及雷帕霉素作用的实验组，将对照组和实验组经不同剂量射线作用；用 MTT 实验（噻唑蓝）及克隆形成能力检测细胞增殖能力，流式细胞仪检测细胞凋亡的变化。结果：雷帕霉素药物作用抑制了 mTOR 信号通路及下游分子的表达，经不同剂量射线照射后细胞的增殖能力和克隆形成能力显著低于对照组，凋亡显著高于对照组。结论：抑制 mTOR 信号通路可以增加非小细胞肺癌对放疗的敏感性。
목적：연구 mTOR 신호통로여비소세포폐암방료민감성적관계。방법：채용불동제량적뢰파매소억제비소세포폐암세포계 A549중적 mTOR 신호통로；Western blot 검측뢰파매소작용후하유신호분자적표체；장 A549세포분위공백대조조급뢰파매소작용적실험조，장대조조화실험조경불동제량사선작용；용 MTT 실험（새서람）급극륭형성능력검측세포증식능력，류식세포의검측세포조망적변화。결과：뢰파매소약물작용억제료 mTOR 신호통로급하유분자적표체，경불동제량사선조사후세포적증식능력화극륭형성능력현저저우대조조，조망현저고우대조조。결론：억제 mTOR 신호통로가이증가비소세포폐암대방료적민감성。
Objective:To investigate the correlation between the activation of mTOR signal pathway and the radio-sensitivity in non -small cell lung cancer (NSCLC).Methods:mTOR pathway was inhibited by different doses of Rapamycin in NSCLC cell line A549.Downstream signaling molecules were detected by Western blot.A549 was divid-ed into the control group and experimental group which was treated by rapamycin,following with different dose of radi-ation.The cell growth was evaluated by MTT assay and clone formation assay.Apoptosis was determined by FACS (fluorescent -activated cell sorting).Results:Rapamycin suppressed the mTOR signal pathway and the expression of downstream molecules.The cell growth and clone formation ability of experimental group were significantly decreased compared with control group,however,the apoptosis was much more than that in control group.Conclusion:Inhibition of mTOR signaling pathway in NSCLC can enhance the sensitivity of radiotherapy.