CAJ | 학술논문

目的：探讨宫颈液基细胞学联合 HPV16、18在宫颈病变筛查中的临床效果。方法2013年1～12月在我院进行宫颈癌筛查和治疗的600例患者随机分成两组，一组进行宫颈脱落细胞的液基细胞学检测（LCT ）和PCR检测，另一组进行宫颈脱落细胞的液基细胞学检测和 HC‐Ⅱ检测。比较两组的灵敏度和特异度；分析LCT、PCR检测和组织病理学结果的关系等。结果 PCR组的灵敏度和特异度分别为88．5％和74．8％，HC‐Ⅱ的灵敏度和特异度分别为72．7％和78．2％，两组的灵敏度和特异度差异无统计学意义（P＞0．05）。PCR收费较 HC‐Ⅱ低，易于被接受。PCR组 HPV16阳性患者，LCT 检测分级为 ASCUS 以上，且经病理检查结果为 CIN Ⅰ以上占64．3％；其他 HR‐HPV阳性和 HR‐HPV阴性，LCT检测分级为ASCUS以上，且经病理检查结果为CINⅠ以上分别占10．9％和1．4％，LCT不同分级感染 HPV的不同型别差异有统计学意义（χ2＝34．955，P＜0．01）。结论LCT联合PCR检测HPV16和 HPV18可以提高宫颈病变的检出率，有助于宫颈癌的早发现、早诊断和早治疗。
목적：탐토궁경액기세포학연합 HPV16、18재궁경병변사사중적림상효과。방법2013년1～12월재아원진행궁경암사사화치료적600례환자수궤분성량조，일조진행궁경탈락세포적액기세포학검측（LCT ）화PCR검측，령일조진행궁경탈락세포적액기세포학검측화 HC‐Ⅱ검측。비교량조적령민도화특이도；분석LCT、PCR검측화조직병이학결과적관계등。결과 PCR조적령민도화특이도분별위88．5％화74．8％，HC‐Ⅱ적령민도화특이도분별위72．7％화78．2％，량조적령민도화특이도차이무통계학의의（P＞0．05）。PCR수비교 HC‐Ⅱ저，역우피접수。PCR조 HPV16양성환자，LCT 검측분급위 ASCUS 이상，차경병리검사결과위 CIN Ⅰ이상점64．3％；기타 HR‐HPV양성화 HR‐HPV음성，LCT검측분급위ASCUS이상，차경병리검사결과위CINⅠ이상분별점10．9％화1．4％，LCT불동분급감염 HPV적불동형별차이유통계학의의（χ2＝34．955，P＜0．01）。결론LCT연합PCR검측HPV16화 HPV18가이제고궁경병변적검출솔，유조우궁경암적조발현、조진단화조치료。
Objective To explore clinical effect of liquid‐based cytology producer joint PCR of HPV16/18 in cervical cancer screening test .Methods 600 patients who were received cervical cancer screening test in our hospital were divided into two groups randomly from January to December in 2013 .One group patients received liquid‐based cytology(LCT)test and PCR test ,another group received LCT and HC‐Ⅱtest .Sensitivity and specificity were com‐pared in two groups .the relationship among LCT ,PCR and tissue of pathology were analyzed .Results Sensitivity and specificity were 88 .5% and 74 .8% respectively in PCR group while which were 72 .7% and 78 .2% respectively in HC‐Ⅱgroup .There was no difference in two groups in this two aspects(P>0 .05) .Cost for receive PCR was chea‐per and more acceptable than HC‐Ⅱgroup .Patients who were HPV16 positive combine with ASCUS above and CINⅠwas 64 .3% while which were 10 .9% and 1 .4% in the patients who were other HR‐HPV positive and HR‐HPV negative .The difference has statistical significance in three levles(χ2 = 34 .955 ,P< 0 .01) .Conclusion LCT com‐bined with PCR of HPV16 and HPV18 could improve cervical cancer screening which could help discovery、diagnosis and treat in early stage of cervical cancer .