CAJ | 학술논문

目的：研究莫西沙星对4株临床耐药葡萄球菌生物膜的体外药效学。方法：采用微量肉汤稀释法测定莫西沙星的最低抑菌浓度（minimal inhibitory concentration，MIC）、最低抑制生物膜浓度（minimal biofilm inhibitory concentration，MBIC）和最低摧毁生物膜浓度（minimal biofilm eradication concentration，MBEC）；测定莫西沙星对细菌生物膜形成量以及存活菌的影响；采用微量稀释棋盘法测定莫西沙星与局部用药瑞他帕林的联合抗生物膜效果。结果：莫西沙星在16~256 mg·L-1的范围内可完全摧毁细菌生物膜；2×MIC显著降低生物膜的形成量；100× MIC可显著降低生物膜存活菌数；与瑞他帕林的联合抗生物膜分数（fractional biofilm inhibitory concentration，FBIC）均小于1.0。结论：莫西沙星对4株耐药葡萄球菌生物膜具有抑制和摧毁作用，而且与局部用药瑞他帕林具有协同作用。
목적：연구막서사성대4주림상내약포도구균생물막적체외약효학。방법：채용미량육탕희석법측정막서사성적최저억균농도（minimal inhibitory concentration，MIC）、최저억제생물막농도（minimal biofilm inhibitory concentration，MBIC）화최저최훼생물막농도（minimal biofilm eradication concentration，MBEC）；측정막서사성대세균생물막형성량이급존활균적영향；채용미량희석기반법측정막서사성여국부용약서타파림적연합항생물막효과。결과：막서사성재16~256 mg·L-1적범위내가완전최훼세균생물막；2×MIC현저강저생물막적형성량；100× MIC가현저강저생물막존활균수；여서타파림적연합항생물막분수（fractional biofilm inhibitory concentration，FBIC）균소우1.0。결론：막서사성대4주내약포도구균생물막구유억제화최훼작용，이차여국부용약서타파림구유협동작용。
Objective: To investigate the antibacterial effect of moxifloxacin against four strains of clinical resistant Staphylococcus biofilms in vitro. Methods: MIC (minimal inhibitory concentrations), MBIC (minimal biofilm inhibitory concentration) and MBEC (minimal biofilm eradication concentration) were examined by microdilution in broth; the percentages of biofilms formed and the viable cells of biofilms were investigated; and the synergy effects against biofilms were evaluated by a microdilution checkerboard method. Results: Moxifloxacin can eradicat biofilms at the concentration of 16-256 mg·L-1; The biofilms formed significantly decreased when moxifloxacin was at 2×MIC; moxifloxacin at 100×MIC caused significant decrease in viable cells of biofilms; the fractional biofilm inhibitory concentrations (FBICs) of moxifloxacin in combination with topical antibiotic retapamulin were all less than 1.0. Conclusions: Moxifloxacin can inhibit and eradicate clinical resistant Staphylococcus biofilms in vitro; synergy was demonstrated for moxifloxacin in combination with topical antibiotic retapamulin against them.