CAJ | 학술논문

目的：探讨白细胞介素－6（IL－6）－572位点单核苷酸多态性与痰瘀阻肺型、肺肾气虚型肺胀相关性。方法选取十堰地区汉族肺肾气虚型肺胀患者78例作为肺肾气虚型组，痰瘀阻肺型肺胀患者64例作为痰瘀阻肺型组，另选择同地区汉族健康受试者182例作为对照组。应用聚合酶链反应、酶切技术对3组IL－6－572位点单核苷酸多态性进行研究，应用夹心酶联免疫吸附法测定血清IL－6水平。结果3组间CC、CG、GG基因型分布情况比较差异均无统计学意义（P均＞0．05）。肺肾气虚型组和痰瘀阻肺型组血清IL－6水平均高于对照组（P均＜0．05），肺肾气虚型组和痰瘀阻肺型组比较差异无统计学意义。结论 IL－6－572位点单核苷酸多态性与痰瘀阻肺型、肺肾气虚型肺胀的发病无明显相关性。
목적：탐토백세포개소－6（IL－6）－572위점단핵감산다태성여담어조폐형、폐신기허형폐창상관성。방법선취십언지구한족폐신기허형폐창환자78례작위폐신기허형조，담어조폐형폐창환자64례작위담어조폐형조，령선택동지구한족건강수시자182례작위대조조。응용취합매련반응、매절기술대3조IL－6－572위점단핵감산다태성진행연구，응용협심매련면역흡부법측정혈청IL－6수평。결과3조간CC、CG、GG기인형분포정황비교차이균무통계학의의（P균＞0．05）。폐신기허형조화담어조폐형조혈청IL－6수평균고우대조조（P균＜0．05），폐신기허형조화담어조폐형조비교차이무통계학의의。결론 IL－6－572위점단핵감산다태성여담어조폐형、폐신기허형폐창적발병무명현상관성。
Objective It is to investigate the correlation of IL -6 -572 single nueleotide polymorphism ( SNP) with lung distension of lung retention of phlegn and blood stasis type and lung -kidney Qi deficiency type .Methods 78 Han patients with lung distension of lung -kidney Qi deficiency type in Shiyan area were selected as lung -kidney Qi deficiency type group, 64 ones of lung retention of phlegn and blood stasis type as lung retention of phlegn and blood stasis type group , 182 healthy ones as control group .The SNP-572 in the IL-6 promoter gene were analyzed by using PCR -RFLP method,the concentrations of serum IL 6 were measured by using ELISA kit .Results There was no statistically differences in the distribu-tion of genotype CC, CG, GG among the three groups (P>0.05).The levels of serum IL-6 in lung-kidney Qi deficiency type group and lung retention of phlegn and blood stasis type group were both higher than that in control group (P<0.05), but there was no significant difference in the level between lung -kidney Qi deficiency type group and lung retention of phlegn and blood stasis type group .Conclusion IL-6 572 SNP was not correlated with the pathogens of COPD patients of lung -kid-ney Qi deficiency type and lung retention of phlegn and blood stasis type .