CAJ | 학술논문

目的：建立高效液相色谱法测定油炸蚕豆中的多菌灵残留的方法。方法试样经1%乙酸-乙腈溶液提取, MCX固相萃取柱净化,甲醇溶解定容,经C18色谱柱分离,以乙腈-水为流动相,在波长274 nm处检测,外标法定量。结果在质量浓度为0.10～4.0 mg/L范围内,峰面积与其浓度的线性关系良好,相关系数为0.9998,方法检出限为0.02 mg/kg,平均回收率为85.2%～95.9%。结论该方法简便、快速准确,适用于油炸蚕豆中多菌灵的测定。
목적：건립고효액상색보법측정유작잠두중적다균령잔류적방법。방법시양경1%을산-을정용액제취, MCX고상췌취주정화,갑순용해정용,경C18색보주분리,이을정-수위류동상,재파장274 nm처검측,외표법정량。결과재질량농도위0.10～4.0 mg/L범위내,봉면적여기농도적선성관계량호,상관계수위0.9998,방법검출한위0.02 mg/kg,평균회수솔위85.2%～95.9%。결론해방법간편、쾌속준학,괄용우유작잠두중다균령적측정。
Objective To study and establish a method for determination of carbendazim residue in fried broad bean by high performance liquid chromatography. Methods The sample was extracted by 1% acetic acid-acetonitrile, cleaned up by MCX SPE, dissolved and volumed by methanol, and then separated by C18 chromatography column, the mobile phase was acetonitrile-water, the detection wavelength was 274 nm, and the quantitave method was the external standard method. Results The linear relationship between peak area and concentration was good over the range of 0.10~4.0 mg/L with the correlation coefficient 0.9998, and the detection limit was 0.020 mg/kg, the average recoveries of the method were ranged from 85.2%~95.9%. Conclusion This method is easier, faster and suitable for the determination of carbendazim in fried broad bean.