食品安全质量检测学报
食品安全質量檢測學報
식품안전질량검측학보
FOOD SAFETY AND QUALITY DETECTION TECHNOLOGY
2015年
8期
3283-3286
,共4页
荧光光谱法%二丁基羟基甲苯%高锰酸钾%氧化
熒光光譜法%二丁基羥基甲苯%高錳痠鉀%氧化
형광광보법%이정기간기갑분%고맹산갑%양화
fluorescent spectrometry%butylated hydroxytoluene%potassium permanganate%oxidation
目的：建立食品中二丁基羟基甲苯(BHT)荧光光谱测定法。方法在KMnO4溶液(pH=2.5)中,温度为75℃的水浴锅中加热50 min后, BHT的氧化产物在λex/λem=292/342 nm处发射强荧光,用荧光光度计测定。结果BHT在1.32~11.01 mg/L范围内,其氧化产物的荧光强度与BHT浓度呈良好的线性关系,相关系数r=0.992,方法检出限为0.01 mg/L,精密度为2.1%。结论该方法有较高灵敏度和较好精密度,可用于食品中BHT的检测。
目的：建立食品中二丁基羥基甲苯(BHT)熒光光譜測定法。方法在KMnO4溶液(pH=2.5)中,溫度為75℃的水浴鍋中加熱50 min後, BHT的氧化產物在λex/λem=292/342 nm處髮射彊熒光,用熒光光度計測定。結果BHT在1.32~11.01 mg/L範圍內,其氧化產物的熒光彊度與BHT濃度呈良好的線性關繫,相關繫數r=0.992,方法檢齣限為0.01 mg/L,精密度為2.1%。結論該方法有較高靈敏度和較好精密度,可用于食品中BHT的檢測。
목적：건립식품중이정기간기갑분(BHT)형광광보측정법。방법재KMnO4용액(pH=2.5)중,온도위75℃적수욕과중가열50 min후, BHT적양화산물재λex/λem=292/342 nm처발사강형광,용형광광도계측정。결과BHT재1.32~11.01 mg/L범위내,기양화산물적형광강도여BHT농도정량호적선성관계,상관계수r=0.992,방법검출한위0.01 mg/L,정밀도위2.1%。결론해방법유교고령민도화교호정밀도,가용우식품중BHT적검측。
Objective To establish a method for the determination of butylated hydroxytoluene (BHT) in food by fluorescent spectrometry. Methods The condition of pretreatment was 1.0×10-4 mol/L concentration of the KMnO4 solution (pH=2.5), 75℃water bath for heating 50 min. After being heated, BHT was oxidated and it emitted strong fluorescence atλex/λem=292/342 nm. The results were determined by fluorimetry. Results There was a good linear relationship between fluorescence intensity and concentration of BHT in the range of 1.32~11.01 mg/L with the correlation coefficient of r=0.992. The limit of detection was 0.01 mg/L, and the relative standard deviation was 2.1%. Conclusion The established method is sensitive and accurate, and it is suitable for the determination of BHT in food.
