CAJ | 학술논문

目的：建立测定醒酒护肝口服液中槲皮素含量的高效液相色谱（HPLC）法。方法色谱柱为 Kromasil C18柱(250 mm ×4.6 mm，5μm)，以甲醇－0.4%磷酸溶液(50：50)为流动相，检测波长360 nm，柱温25℃。结果槲皮素进样量在0.1098~0.5490μg 范围内与峰面积呈良好的线性关系（ r =0.9995）。平均回收率为98.26%，RSD 为0.61%（ n =6）。结论该法操作简便、快速、重复性好，可用于醒酒护肝口服液的质量控制。
목적：건립측정성주호간구복액중곡피소함량적고효액상색보（HPLC）법。방법색보주위 Kromasil C18주(250 mm ×4.6 mm，5μm)，이갑순－0.4%린산용액(50：50)위류동상，검측파장360 nm，주온25℃。결과곡피소진양량재0.1098~0.5490μg 범위내여봉면적정량호적선성관계（ r =0.9995）。평균회수솔위98.26%，RSD 위0.61%（ n =6）。결론해법조작간편、쾌속、중복성호，가용우성주호간구복액적질량공제。
Objective To investigate quantitative determination method of quercetin in Xingjiuhugan oral solution to provide identifiable ground for the foundation of quality standards. Methods Octadecylsilane chemically bonded silica column was adopted with methanol -0. 4% phosphate(50 : 50) as flowing phase, testing wave 360 nm, column temperature 25 ℃ . Results A good linear relationship of Quercetin showed between 0. 109 8 - 0. 549 0 μg( r = 0. 999 5). Lower limit of average recovery was 98. 26% ,RSD = 0. 61% ( n = 6). Conclusion This method is simple, rapid, reproducible and can be used for the quality control of Xingjiuhugan oral liquid.