CAJ | 학술논문

目的：建立一种快速、简便测定茶氨酸提取物中咖啡因的液相色谱方法。方法样品经过前处理,以1-癸烷磺酸钠溶液(1.22 g→850 mL)+乙腈+磷酸=850+150+1(V：V：V)为流动相,流速1.0 mL/min,柱温40℃,进样量10μL,等度洗脱,经Agilent C18柱分离,于280 nm波长处检测,以外标法定量。结果用本方法进行检测,能很好的分离咖啡因,在浓度0.001 mg/mL至0.05 mg/mL之间呈现良好的线性关系,方法的相关系数可达1.000,平均回收率为100.2,在95%~105%之间,相对标准偏差(RSD)为1.0%,定量限为92.78μg/g,检出限为29.03μg/g。结论该方法耗时短、操作简便、准确、重现性好、分离效果明显。可以应用于茶氨酸提取物中咖啡因含量的测定。
목적：건립일충쾌속、간편측정다안산제취물중가배인적액상색보방법。방법양품경과전처리,이1-계완광산납용액(1.22 g→850 mL)+을정+린산=850+150+1(V：V：V)위류동상,류속1.0 mL/min,주온40℃,진양량10μL,등도세탈,경Agilent C18주분리,우280 nm파장처검측,이외표법정량。결과용본방법진행검측,능흔호적분리가배인,재농도0.001 mg/mL지0.05 mg/mL지간정현량호적선성관계,방법적상관계수가체1.000,평균회수솔위100.2,재95%~105%지간,상대표준편차(RSD)위1.0%,정량한위92.78μg/g,검출한위29.03μg/g。결론해방법모시단、조작간편、준학、중현성호、분리효과명현。가이응용우다안산제취물중가배인함량적측정。
Objective To establish a quick and simple method for the determination of caffeine in theanine extracts by high performance liquid chromatography (HPLC). Methods Samples were pretreated and were separated by agilent C18 column with gradient elution by a mobile phase of 1-decane sulfonic acid sodium solution(1.22 g→850 mL)-acetonitrile-phosphoric acid with 850+150+1(V:V:V), the flow rate at 1.0 mL/min, column temperature at 30℃, injection volume at 10μL, and detected at 280 nm, then quantified by external standard method. Results Caffeine can be separated well and had a good linearity in the range of 0.001~0.05 mg/mL (r=1.000), the recovery was 95%~105%, and RSD was 1.0%;the limit of quantitation was 92.78μg/g, and the limit of detection was 29.03μg/g. Conclusion The method is quick, simple and accurate, and has a good reproducibility and clear separation. It can be applied for the determination of caffeine in tea theanine extracts.