中国药业
中國藥業
중국약업
CHINA PHARMACEUTICALS
2015年
15期
4-5
,共2页
万古霉素%反相高效液相色谱法%血药浓度%方法改进
萬古黴素%反相高效液相色譜法%血藥濃度%方法改進
만고매소%반상고효액상색보법%혈약농도%방법개진
vancomycin%RP - HPLC%plasma drug concentration%modified method
目的：改进测定万古霉素血药浓度的反相高效液相色谱（RP － HPLC）法。方法采用外标法。血样处理，血浆经30%硫酸锌、50℃水浴孵育10 min 沉淀蛋白，色谱柱为 Hypersil ODS －2 C18柱（250 mm ×4.6 mm，5μm），流动相为25 mmol / L 磷酸二氢钾溶液（用磷酸调 pH 至3.2）－甲醇（83：17），柱温25℃，流速1.0 mL / min，检测波长230 nm，进样体积20μL。结果万古霉素检测质量浓度在1~100 mg / L 范围内与峰面积呈良好线性关系（ r =0.9996），检测限（ LOD）为0.2 mg / L。平均相对回收率为101.17%，RSD 为5.20%( n =15)，日内与日间 RSD 均小于6.0%。结论该法简单灵敏、无杂质干扰、线性范围宽、成本较低，适用于基层医院的治疗药物监测。
目的：改進測定萬古黴素血藥濃度的反相高效液相色譜（RP － HPLC）法。方法採用外標法。血樣處理，血漿經30%硫痠鋅、50℃水浴孵育10 min 沉澱蛋白，色譜柱為 Hypersil ODS －2 C18柱（250 mm ×4.6 mm，5μm），流動相為25 mmol / L 燐痠二氫鉀溶液（用燐痠調 pH 至3.2）－甲醇（83：17），柱溫25℃，流速1.0 mL / min，檢測波長230 nm，進樣體積20μL。結果萬古黴素檢測質量濃度在1~100 mg / L 範圍內與峰麵積呈良好線性關繫（ r =0.9996），檢測限（ LOD）為0.2 mg / L。平均相對迴收率為101.17%，RSD 為5.20%( n =15)，日內與日間 RSD 均小于6.0%。結論該法簡單靈敏、無雜質榦擾、線性範圍寬、成本較低，適用于基層醫院的治療藥物鑑測。
목적：개진측정만고매소혈약농도적반상고효액상색보（RP － HPLC）법。방법채용외표법。혈양처리，혈장경30%류산자、50℃수욕부육10 min 침정단백，색보주위 Hypersil ODS －2 C18주（250 mm ×4.6 mm，5μm），류동상위25 mmol / L 린산이경갑용액（용린산조 pH 지3.2）－갑순（83：17），주온25℃，류속1.0 mL / min，검측파장230 nm，진양체적20μL。결과만고매소검측질량농도재1~100 mg / L 범위내여봉면적정량호선성관계（ r =0.9996），검측한（ LOD）위0.2 mg / L。평균상대회수솔위101.17%，RSD 위5.20%( n =15)，일내여일간 RSD 균소우6.0%。결론해법간단령민、무잡질간우、선성범위관、성본교저，괄용우기층의원적치료약물감측。
Objective To determine the concentration of vancomycin in human plasma using a modified RP - HPLC method. Methods The external standard method was adopted for the assay. The plasma samples were purified via protein precipitation with 30% ZnSO4 and incubated in water at 50 ℃ for 10 min. The separation was performed on Thermo Hypersil ODS - 2 C18 column(250 mm × 4. 6 mm, 5 μm) with a mobile phase of 25 mmol / L potassium dihydrogen phosphate solution(adjusted to pH = 3. 2 with phosphoric acid) -methanol(83 : 17). The column temperature was 25 ℃ , with a flow rate of 1. 0 ml / min. The detective wavelength was 230 nm and the injection volumn was 20 μL. Results The peak shape was good, and no endogenous interfering peaks were observed in the blank plas-ma. The calibration curve was linear in the concentration range of 1 - 100 mg / L( r = 0. 999 6) and the limit of detection ( LOD) was 0. 2 mg / L. The average relative recoveries was 101. 17% respectively at low, middle and high concentrations, the RSDs was 5. 20%( n = 15), all less than 6. 0% . Conclusion The method is simple, sensitive and specific with wide concentration rage and low cost. It is a reliable method for therapeutic drug monitoring of vancomycin.
