CAJ | 학술논문

目的：建立高效液相色谱-蒸发光散射检测(HPLC-ELSD)法,快速洗脱分离出蔗果三糖、蔗果四糖、蔗果五糖,测定益生菌粉中低聚果糖的含量。方法样品经过前处理,以乙腈和水为流动相,等度洗脱,经XAmide (4.6 mm×250 mm,5μm)中性酰胺色谱柱分离,采用蒸发激光检测器检测。结果蔗果三糖、蔗果四糖、蔗果五糖线性范围分别在0.2671～3.168 mg/mL、0.2429～2.8215 mg/mL、0.1617～3.544 mg/mL,线性相关系数r＞0.99。3种主要组成的回收率在97.0%～105.0%之间。结论该方法可有效分离出蔗果三糖、蔗果四糖、蔗果五糖,推广性高、快速、简便、准确,可用于益生菌粉中低聚果糖含量的测定。
목적：건립고효액상색보-증발광산사검측(HPLC-ELSD)법,쾌속세탈분리출자과삼당、자과사당、자과오당,측정익생균분중저취과당적함량。방법양품경과전처리,이을정화수위류동상,등도세탈,경XAmide (4.6 mm×250 mm,5μm)중성선알색보주분리,채용증발격광검측기검측。결과자과삼당、자과사당、자과오당선성범위분별재0.2671～3.168 mg/mL、0.2429～2.8215 mg/mL、0.1617～3.544 mg/mL,선성상관계수r＞0.99。3충주요조성적회수솔재97.0%～105.0%지간。결론해방법가유효분리출자과삼당、자과사당、자과오당,추엄성고、쾌속、간편、준학,가용우익생균분중저취과당함량적측정。
Objective To establish a rapid determination for the rapid separate kestose, nystose and 1F-fructofuranosyl nystose by high performance liquid chromatography-evaporative light-scattering detector (HPLC-ELSD) method, and the concentration of fruto-oligosaccharides can be determined in probiotics powder. Methods After pretreatment of samples, acetonitrile and water as the mobile phase, while the elution method was gradient elution, the samples were separated by XAmide (4.6 mm×250 mm,5 μm) neutral amide chromatographic column, and detected by evaporative laser detector. Results The linear ranges of kestose, nystose and 1F-fructofuranosyl nystose were from 0.2671 to 3.168 mg/mL, 0.2429 to 2.8215 mg/mL and 0.1617 to 3.544 mg/mL, respectively, and the linear correlation coefficient r>0.99. The recovery rate of the 3 compositions was between 97.0%~105.0%. Conclusion This method can effectively separate the kestose, nystose and 1F-fructofuranosyl nystose. It is a high generalization, rapid, simple and accurate method, and can be applied for the determination of fruto-oligosaccharides in probiotics powder.