食品安全质量检测学报
食品安全質量檢測學報
식품안전질량검측학보
FOOD SAFETY AND QUALITY DETECTION TECHNOLOGY
2015年
8期
3070-3074
,共5页
氨基葡萄糖硫酸钾盐%柱前在线衍生%邻苯二甲醛衍生试剂%反相高效液相色谱法
氨基葡萄糖硫痠鉀鹽%柱前在線衍生%鄰苯二甲醛衍生試劑%反相高效液相色譜法
안기포도당류산갑염%주전재선연생%린분이갑철연생시제%반상고효액상색보법
N-Sulfo-glucosamine potassium salt%online pre-column derivatization%phthalic dicarboxaldehyde derived reagents%reversed-phase high performance liquid chromatography
目的：建立一种反相高效液相色谱柱前在线衍生法测定保健品中氨基葡萄糖硫酸钾盐的含量。方法利用邻苯二甲醛衍生试剂与氨基葡萄糖硫酸钾盐中的氨基进行柱前在线衍生化反应,采用 C18色谱柱分离,以0.68%醋酸缓冲液：甲醇(V：V=82：18)为流动相,流速1.0 mL/min,检测波长340 nm。结果 D-盐酸氨基葡萄糖浓度在0.052～1.045 mg/mL之间呈现良好的线性,平均回收率为97.5%, RSD%=1.2(n=9)。结论该方法推广性高、简便、准确,可用于制剂及保健品中氨基葡萄糖硫酸钾盐的含量测定。
目的：建立一種反相高效液相色譜柱前在線衍生法測定保健品中氨基葡萄糖硫痠鉀鹽的含量。方法利用鄰苯二甲醛衍生試劑與氨基葡萄糖硫痠鉀鹽中的氨基進行柱前在線衍生化反應,採用 C18色譜柱分離,以0.68%醋痠緩遲液：甲醇(V：V=82：18)為流動相,流速1.0 mL/min,檢測波長340 nm。結果 D-鹽痠氨基葡萄糖濃度在0.052～1.045 mg/mL之間呈現良好的線性,平均迴收率為97.5%, RSD%=1.2(n=9)。結論該方法推廣性高、簡便、準確,可用于製劑及保健品中氨基葡萄糖硫痠鉀鹽的含量測定。
목적：건립일충반상고효액상색보주전재선연생법측정보건품중안기포도당류산갑염적함량。방법이용린분이갑철연생시제여안기포도당류산갑염중적안기진행주전재선연생화반응,채용 C18색보주분리,이0.68%작산완충액：갑순(V：V=82：18)위류동상,류속1.0 mL/min,검측파장340 nm。결과 D-염산안기포도당농도재0.052～1.045 mg/mL지간정현량호적선성,평균회수솔위97.5%, RSD%=1.2(n=9)。결론해방법추엄성고、간편、준학,가용우제제급보건품중안기포도당류산갑염적함량측정。
Objective To establish a method for the determination of N-Sulfo-glucosamine potassium salt by reversed-phase high performance liquid chromatography (RP-HPLC) with online pre-column derivation in health food. Methods Online pre-column derivation were reacted by using phthalic dicarboxaldehyde (OPA) derived reagents and amino of N-Sulfo-glucosamine potassium salt. The products were separated by C18 column with 0.68%acetic acid buffer:methanol (V:V=82:18) as mobile phase, the flow rate of 1.0 mL/min, and detection wavelength of 340 nm. Results D-glucosamine hydrochloride concentration had a good linearity in the range of 0.052~1.045 mg/mL. The average recovery rate was 97.5%, RSD%=1.2 (n=9). Conclusion The method is high, simple and accurate, and can be used to determine the content of N-Sulfo-glucosamine potassium salt in pharmaceutics and health food.
