食品安全质量检测学报
食品安全質量檢測學報
식품안전질량검측학보
FOOD SAFETY AND QUALITY DETECTION TECHNOLOGY
2015年
8期
3060-3063
,共4页
李跃彬%俞金标%吴斯燕%植爱萍
李躍彬%俞金標%吳斯燕%植愛萍
리약빈%유금표%오사연%식애평
总黄酮%苯%石油醚(30~60°)
總黃酮%苯%石油醚(30~60°)
총황동%분%석유미(30~60°)
total flavonoids%benzene%petroleum ether(30~60°)
目的：建立以石油醚洗脱杂质测定巴西绿蜂胶胶囊中总黄酮的方法。方法样品经乙醇提取,用聚酰胺粉吸附,分别用石油醚、苯不同溶剂洗去杂质；经甲醇洗脱后,在波长360 nm处,用分光光度法测定巴西绿蜂胶中总黄酮的含量。结果采用石油醚作为洗脱剂,总黄酮含量在1.9897～23.8761μg/mL范围内,与吸光度值呈良好的线性关系,浓度-吸光度回归方程为 Y=0.02976X-0.00741,相关系数r2=0.99954。方法精密度为1.0%,平均回收率为96.5%。与用苯洗脱杂质结果比较,两者无显著性差异。结论该方法操作简便,重现性及稳定性好,线性范围宽,减少了苯对检测人员和环境的污染,可用于保健食品中总黄酮的测定。
目的：建立以石油醚洗脫雜質測定巴西綠蜂膠膠囊中總黃酮的方法。方法樣品經乙醇提取,用聚酰胺粉吸附,分彆用石油醚、苯不同溶劑洗去雜質；經甲醇洗脫後,在波長360 nm處,用分光光度法測定巴西綠蜂膠中總黃酮的含量。結果採用石油醚作為洗脫劑,總黃酮含量在1.9897～23.8761μg/mL範圍內,與吸光度值呈良好的線性關繫,濃度-吸光度迴歸方程為 Y=0.02976X-0.00741,相關繫數r2=0.99954。方法精密度為1.0%,平均迴收率為96.5%。與用苯洗脫雜質結果比較,兩者無顯著性差異。結論該方法操作簡便,重現性及穩定性好,線性範圍寬,減少瞭苯對檢測人員和環境的汙染,可用于保健食品中總黃酮的測定。
목적：건립이석유미세탈잡질측정파서록봉효효낭중총황동적방법。방법양품경을순제취,용취선알분흡부,분별용석유미、분불동용제세거잡질；경갑순세탈후,재파장360 nm처,용분광광도법측정파서록봉효중총황동적함량。결과채용석유미작위세탈제,총황동함량재1.9897～23.8761μg/mL범위내,여흡광도치정량호적선성관계,농도-흡광도회귀방정위 Y=0.02976X-0.00741,상관계수r2=0.99954。방법정밀도위1.0%,평균회수솔위96.5%。여용분세탈잡질결과비교,량자무현저성차이。결론해방법조작간편,중현성급은정성호,선성범위관,감소료분대검측인원화배경적오염,가용우보건식품중총황동적측정。
Objective To establish a method for determining flavonoids in Brazil green propolis capsule. Methods The total flavonoids in Brazil green propolis capsule were determined by spectrophotometry after the samples were distilled by methanol and purified by petroleum ether. The detection wavelength was 360 nm. Results The calibration curve of flavonoids content and absorbance had a good linearity in the range of 1.9897~23.8761 μg/mL (r2=99954). The relative standard deviations were 1.0%, and the recoveries were 96.5%. The results had no obvious difference which purified by petroleum ether instead of benzene. Conclusion The method is simple, stable and accurate, and it can be applied successfully to determine flavonoids in health food.
