食品安全质量检测学报
食品安全質量檢測學報
식품안전질량검측학보
FOOD SAFETY AND QUALITY DETECTION TECHNOLOGY
2015年
8期
3045-3049
,共5页
微生物法%生物素%多种维生素片
微生物法%生物素%多種維生素片
미생물법%생물소%다충유생소편
microbial method%biotin,multi-vitamins tablets
目的：利用微生物法测定多种维生素片中生物素的含量。方法在GB 5413.19-2010分析方法的基础上,通过将乳酸杆菌培养基改成MRS肉汤培养基来制备菌悬液,样品经过65℃~70℃水浴超声提取,在630 nm 波长下用酶标仪测定培养液吸光度,对生物素含量进行定量检测。与国标法进行标准曲线、精密度、准确度对比试验,以及对新建立的方法进行加标回收试验。结果生物素浓度在0.01~0.1 ng/mL范围内与吸光度呈现良好的二次曲线关系,相关系数r2>0.999,精密度和准确度良好。在80%、100%、120%添加水平下,生物素的回收率分别为99.8%、96.1%、97.2%。结论方法操作简单、灵敏度高、处理量大,适合生产企业的多种维生素片的大批量质量控制和检测。
目的：利用微生物法測定多種維生素片中生物素的含量。方法在GB 5413.19-2010分析方法的基礎上,通過將乳痠桿菌培養基改成MRS肉湯培養基來製備菌懸液,樣品經過65℃~70℃水浴超聲提取,在630 nm 波長下用酶標儀測定培養液吸光度,對生物素含量進行定量檢測。與國標法進行標準麯線、精密度、準確度對比試驗,以及對新建立的方法進行加標迴收試驗。結果生物素濃度在0.01~0.1 ng/mL範圍內與吸光度呈現良好的二次麯線關繫,相關繫數r2>0.999,精密度和準確度良好。在80%、100%、120%添加水平下,生物素的迴收率分彆為99.8%、96.1%、97.2%。結論方法操作簡單、靈敏度高、處理量大,適閤生產企業的多種維生素片的大批量質量控製和檢測。
목적：이용미생물법측정다충유생소편중생물소적함량。방법재GB 5413.19-2010분석방법적기출상,통과장유산간균배양기개성MRS육탕배양기래제비균현액,양품경과65℃~70℃수욕초성제취,재630 nm 파장하용매표의측정배양액흡광도,대생물소함량진행정량검측。여국표법진행표준곡선、정밀도、준학도대비시험,이급대신건립적방법진행가표회수시험。결과생물소농도재0.01~0.1 ng/mL범위내여흡광도정현량호적이차곡선관계,상관계수r2>0.999,정밀도화준학도량호。재80%、100%、120%첨가수평하,생물소적회수솔분별위99.8%、96.1%、97.2%。결론방법조작간단、령민도고、처리량대,괄합생산기업적다충유생소편적대비량질량공제화검측。
Objective To determine the content of biotin in multi-vitamins tablets by microbial assay. Methods On the basis of GB 5413.19 2010 analysis method, bacteria suspension were prepared by lactic acid bacteria culture medium to (MRS) broth culture medium, the sample was extracted by water bath ultrasonic after 65℃~70℃;then culture medium absorbance was measured by absorbance enzyme standard instrument under the 630 nm, and to quantitatively detect the content of biotin. The standard curve, precision and accuracy of contrast test were compared with the national standard method, and the new method had carried on the standard addition recovery test. Results Biotin concentration within the scope of 0.01 to 0.1 ng/mL and absorbance had a good conical relationship, the correlation coefficient r2>0.999, and had a good precision and accuracy. The recovery rates of biotin were 99.8%, 96.1%and 97.2%under adding level 80%, 100%, and 120%, respectively. Conclusion The method is simple, high sensitivity and large quantity, and it is suitable for the mass production enterprises of various vitamin pills quality control and testing.
