中国药师
中國藥師
중국약사
CHINA PHARMACIST
2015年
8期
1397-1399
,共3页
毛菊华%王伟影%陈张金%薛薇
毛菊華%王偉影%陳張金%薛薇
모국화%왕위영%진장금%설미
桑叶%芦丁%异槲皮苷%含量测定
桑葉%蘆丁%異槲皮苷%含量測定
상협%호정%이곡피감%함량측정
Mori Folium%Rutin%Isoquercitrin%Assay
目的::对《中国药典》中桑叶含量测定项下方法进行改进。方法:采用HPLC法,Agilent Zorbax SB-C18色谱柱(250 mm ×4.6 mm,5μm);流动相为乙腈-0.2%磷酸水溶液(梯度洗脱),流速为1.0 ml·min-1;检测波长为354 nm;柱温为30℃。结果:《中国药典》2010年版桑叶含量测定项下测得的芦丁含量实为芦丁和异槲皮苷的含量之和,优化后方法可分离该两种化合物,且芦丁和异槲皮苷分别在2.76~27.60μg·ml-1(r=0.9999)和4.74~47.39μg·ml-1(r=0.9998)范围内与峰面积呈良好的线性关系;平均加样回收率分别为100.31%(RSD=0.83%)和100.32%(RSD=1.04%)(n=6)。结论:优化后方法简便、稳定、重复性好,可用于桑叶的质量控制。
目的::對《中國藥典》中桑葉含量測定項下方法進行改進。方法:採用HPLC法,Agilent Zorbax SB-C18色譜柱(250 mm ×4.6 mm,5μm);流動相為乙腈-0.2%燐痠水溶液(梯度洗脫),流速為1.0 ml·min-1;檢測波長為354 nm;柱溫為30℃。結果:《中國藥典》2010年版桑葉含量測定項下測得的蘆丁含量實為蘆丁和異槲皮苷的含量之和,優化後方法可分離該兩種化閤物,且蘆丁和異槲皮苷分彆在2.76~27.60μg·ml-1(r=0.9999)和4.74~47.39μg·ml-1(r=0.9998)範圍內與峰麵積呈良好的線性關繫;平均加樣迴收率分彆為100.31%(RSD=0.83%)和100.32%(RSD=1.04%)(n=6)。結論:優化後方法簡便、穩定、重複性好,可用于桑葉的質量控製。
목적::대《중국약전》중상협함량측정항하방법진행개진。방법:채용HPLC법,Agilent Zorbax SB-C18색보주(250 mm ×4.6 mm,5μm);류동상위을정-0.2%린산수용액(제도세탈),류속위1.0 ml·min-1;검측파장위354 nm;주온위30℃。결과:《중국약전》2010년판상협함량측정항하측득적호정함량실위호정화이곡피감적함량지화,우화후방법가분리해량충화합물,차호정화이곡피감분별재2.76~27.60μg·ml-1(r=0.9999)화4.74~47.39μg·ml-1(r=0.9998)범위내여봉면적정량호적선성관계;평균가양회수솔분별위100.31%(RSD=0.83%)화100.32%(RSD=1.04%)(n=6)。결론:우화후방법간편、은정、중복성호,가용우상협적질량공제。
To optimize the extract method and chromatographic conditions for the determination of rutin in Mori Folium in Chinese pharmacopoeia. Methods:The HPLC analysis was performed on a ZORBAX SB-C18 (250 mm × 4. 6 mm, 5 μm) column. The mobile phase was acetonitrile-0. 2% phosphorice acid solution with gradient elution and the flow rate was 1. 0 ml·min-1 . The de-tection wavelength was 354 nm, and the column temperature was 30 ℃. Results: The content of rutin determined by the method in Chinese pharmacopoeia was actually the total contents of rutin and isoquercitrin, while the optimized method could separate the two components effectively, and the good linearity of rutin and isoquercitrin was within the range of 2.76-27.60 μg·ml-1(r=0.999 9) and 4. 74-47. 39 μg·ml-1(r=0. 999 8), respectively, and the average recovery was 100. 31% (RSD=0. 83%) and 100. 32%, re-spectively (RSD=1. 04%, n=6). Conclusion:The optimized method is simple, stable and reproducible,which can be used in the quality control of Mori Folium.