CAJ | 학술논문

目的：通过羧甲基化及金属络合对苦瓜多糖进行分子修饰,比较不同修饰方法对其抗氧化活性产生的影响。方法采用水提醇沉法分离制备苦瓜粗多糖(Momordica charantia polysaccharide , MCP),以DEAE-52离子交换层析梯度洗脱获得4种苦瓜多糖组分(MCPⅠ, MCPⅠ, MCPⅠ, MCPⅠ)。将其中活性较高组分MCPⅠ进行羧甲基化及金属络合获得苦瓜多糖衍生物CMMCPⅠ、MCPⅠ-Ca(Ⅰ),并以Fenton法检测其·OH清除率。结果苦瓜多糖活性组分MCPⅠ及其衍生物MCPⅠ-Ca(Ⅰ)、CMMCPⅠ均具有抗氧化活性,在质量浓度为0.7 mg/mL时,其对·OH的清除率分别为58%、82%和93%, SC50值均在0.6 mg/mL以下,其中,苦瓜多糖羧甲基化衍生物的抗氧化活性最高。结论通过羧甲基化及金属络合可增强苦瓜多糖生物活性。
목적：통과최갑기화급금속락합대고과다당진행분자수식,비교불동수식방법대기항양화활성산생적영향。방법채용수제순침법분리제비고과조다당(Momordica charantia polysaccharide , MCP),이DEAE-52리자교환층석제도세탈획득4충고과다당조분(MCPⅠ, MCPⅠ, MCPⅠ, MCPⅠ)。장기중활성교고조분MCPⅠ진행최갑기화급금속락합획득고과다당연생물CMMCPⅠ、MCPⅠ-Ca(Ⅰ),병이Fenton법검측기·OH청제솔。결과고과다당활성조분MCPⅠ급기연생물MCPⅠ-Ca(Ⅰ)、CMMCPⅠ균구유항양화활성,재질량농도위0.7 mg/mL시,기대·OH적청제솔분별위58%、82%화93%, SC50치균재0.6 mg/mL이하,기중,고과다당최갑기화연생물적항양화활성최고。결론통과최갑기화급금속락합가증강고과다당생물활성。
Objective To investigate the antioxidant activities of modified polysaccharides, which was embellished by carboxy methylated and metal ions. Methods Water soluble crude polysaccharide was extracted from Momordica charantia L. with hot water and precipitated by ethanol, then purified by DEAE-cellulose ion-exchange chromatography with NaCl gradient elution to obtain 4 kinds of polysaccharide components (MCPⅠ, MCPⅠ, MCPⅠ, MCPⅠ). The antioxidant activities of MCPⅠ were tested by determining scavenging rates of the fraction on ·OH in vitro. The calcium-polysaccharide complex was synthesized when chelated with Ca2+, coded as MCPⅠ-Ca(Ⅰ) . The carboxy methylated polysaccharide was named as CMMCPⅠ. Results The MCPⅠ, MCPⅠ-Ca(Ⅰ) and CMMCPⅠ showed obvious antioxidant activities to ·OH. Their scavenging rates were 58%, 82% and 93% at a mass concentration of 0.7 mg/mL, respectively. The SC50 values in the study were less than 0.6 mg/mL. Furthermore, CMCPⅠ exhibited the strongest antioxidant activities among them. Conclusion The current study suggests that carboxy methylated and metal ions can improve polysaccharide bearing excellent antioxidant activities.