CAJ | 학술논문

为获得高抗氧化活性的金枪鱼骨粉酶解液。用中性蛋白酶、碱性蛋白酶、木瓜蛋白酶、胃蛋白酶4种蛋白酶对鱼骨粉进行酶解，以酶解液的DPPH自由基清除活性为主要指标，水解度为辅助指标进行分析，筛选出试验最适水解酶为中性蛋白酶。采用响应面设计方法对鱼骨粉酶解工艺进行优化。结果表明，骨粉最佳酶解工艺参数为：酶解温度56.22℃，酶解时间1.88 h，pH 6.15，液料比20.19∶1（mL∶g）。该条件下的鱼骨粉酶解液蛋白质浓度为9.30 mg/mL，水解度为12.34%， DPPH清除率为94.97%，羟基（·OH）自由基清除率为97.89%。骨粉酶解液显示出较强的抗氧化活性，且优于同浓度条件下的VC液抗氧化活性。
위획득고항양화활성적금창어골분매해액。용중성단백매、감성단백매、목과단백매、위단백매4충단백매대어골분진행매해，이매해액적DPPH자유기청제활성위주요지표，수해도위보조지표진행분석，사선출시험최괄수해매위중성단백매。채용향응면설계방법대어골분매해공예진행우화。결과표명，골분최가매해공예삼수위：매해온도56.22℃，매해시간1.88 h，pH 6.15，액료비20.19∶1（mL∶g）。해조건하적어골분매해액단백질농도위9.30 mg/mL，수해도위12.34%， DPPH청제솔위94.97%，간기（·OH）자유기청제솔위97.89%。골분매해액현시출교강적항양화활성，차우우동농도조건하적VC액항양화활성。
In order to obtain enzymatic hydrolysates with high antioxidative ability from tuna bone. Taking scavenging activity on DPPH free radicals and the hydroxyl free radicals as evaluating indexex. Antioxidative substances were respectively extracted with Neutrase, Alcalase, Papain and Pepsin, among which Neutrase was showed to be optimal. Single facter and Response surface methodology (RSM) were used to optimize hydrolysis conditions. The test showed that, the optimal hydrolysis conditions were as follows:hydrolysis temperature of 56.22℃, hydrolysis time of 1.88 h, pH of 6.15, liquid-material ratio of 20.19∶1(mL∶g). Under this condition, the protein concentration of enzymatic hydrolysates, the hydrolysis degree, the scavenging rate on DPPH free radicals and hydroxyl free radicals were be 9.30 mg/mL, 12.34 %, 94.97 %, 97.89 %, respectively. The antioxidative activity of enzymatic hydrolysates was higher than that of ascorbic acid at the same concentration.