中国药师
中國藥師
중국약사
CHINA PHARMACIST
2015年
8期
1312-1314,1315
,共4页
毛细管电泳%复方磷酸可待因口服制剂%含量测定
毛細管電泳%複方燐痠可待因口服製劑%含量測定
모세관전영%복방린산가대인구복제제%함량측정
Capillary electrophoresis%Compound codeine phosphate oral preparations%Determination
目的：：建立毛细管电泳法( CE)法同时测定复方磷酸可待因口服制剂中磷酸可待因、盐酸麻黄碱、马来酸溴苯那敏、马来酸氯苯那敏、愈创甘油醚等有效成分的含量。方法：色谱柱为贝克曼eCAPTM未涂层石英毛细管柱(50 cm ×75μm)；分离电压为10 kV；缓冲溶液为20 mmol·L-1磷酸盐缓冲液(pH 7.5)；检测波长为214 nm。结果：各测定组分在考察浓度范围内线性关系良好(r≥0.995)，回收率均≥96%。结论：该方法可用于不同复方磷酸可待因口服制剂中有效成分的含量测定，简单有效，具有通用性，便于快速有效的市场监督。
目的：：建立毛細管電泳法( CE)法同時測定複方燐痠可待因口服製劑中燐痠可待因、鹽痠痳黃堿、馬來痠溴苯那敏、馬來痠氯苯那敏、愈創甘油醚等有效成分的含量。方法：色譜柱為貝剋曼eCAPTM未塗層石英毛細管柱(50 cm ×75μm)；分離電壓為10 kV；緩遲溶液為20 mmol·L-1燐痠鹽緩遲液(pH 7.5)；檢測波長為214 nm。結果：各測定組分在攷察濃度範圍內線性關繫良好(r≥0.995)，迴收率均≥96%。結論：該方法可用于不同複方燐痠可待因口服製劑中有效成分的含量測定，簡單有效，具有通用性，便于快速有效的市場鑑督。
목적：：건립모세관전영법( CE)법동시측정복방린산가대인구복제제중린산가대인、염산마황감、마래산추분나민、마래산록분나민、유창감유미등유효성분적함량。방법：색보주위패극만eCAPTM미도층석영모세관주(50 cm ×75μm)；분리전압위10 kV；완충용액위20 mmol·L-1린산염완충액(pH 7.5)；검측파장위214 nm。결과：각측정조분재고찰농도범위내선성관계량호(r≥0.995)，회수솔균≥96%。결론：해방법가용우불동복방린산가대인구복제제중유효성분적함량측정，간단유효，구유통용성，편우쾌속유효적시장감독。
To establish a method for the simultaneous determination of the active ingredients ( codeine phosphate, brompheniramine maleate, chlorpheniramine maleate, ephedrine hydrochloride and guaifenesin) in compound codeine phosphate oral preparations by capillary electrophoresis ( CE) . Methods:The method employed an uncoated capillary column ( eCAPTM ) from Beck-mann company (50 cm × 75 μm);the electrophoresis voltage was at 10 kV;20 mmol·L-1 phosphate buffer solution (pH 7. 5) was used;the UV measurement was at the wavelength of 214 nm. Results: The studied components had good linear ranges (r≥0. 995) within the range of the investigated concentrations. The recovery was no less than 96%. Conclusion:The presented method can be ap-plied in the content determination of active ingredients in compound codeine phosphate oral preparations from different enterprises. It is simple, efficient and universal, which facilitates the market supervision in a fast and valid manner.
