CAJ | 학술논문

目的：：研究建立平疣颗粒的质量控制标准。方法：采用薄层色谱法对方中赤芍、红花、薏苡仁、香附进行定性鉴别；采用高效液相色谱法测定赤芍中芍药苷的含量：色谱柱为Lichrospher-C18(250 mm ×4.6 mm,5μm),流动相为甲醇-0.2%醋酸(24∶76),检测波长为232 nm。结果：薄层色谱鉴别斑点清晰、阴性对照无干扰；芍药苷在6.560~104.920μg·ml-1的范围内呈良好的线性关系(r=0.9999),平均回收率为98.77%,RSD为2.73%(n=9)。结论：所建立的定性鉴别方法专属性强,重复性好,定量方法操作简便,准确可靠,可用于平疣颗粒的质量控制。
목적：：연구건립평우과립적질량공제표준。방법：채용박층색보법대방중적작、홍화、의이인、향부진행정성감별；채용고효액상색보법측정적작중작약감적함량：색보주위Lichrospher-C18(250 mm ×4.6 mm,5μm),류동상위갑순-0.2%작산(24∶76),검측파장위232 nm。결과：박층색보감별반점청석、음성대조무간우；작약감재6.560~104.920μg·ml-1적범위내정량호적선성관계(r=0.9999),평균회수솔위98.77%,RSD위2.73%(n=9)。결론：소건립적정성감별방법전속성강,중복성호,정량방법조작간편,준학가고,가용우평우과립적질량공제。
To establish the quality standard for Pingyou granules. Methods: Paeoniae radix, Carthamus tinctorius, Coicis semen and Nutgrass galingale rhizome were qualitatively identified by TLC. The content of peoniflorin was determined by HPLC. The separation was performed on a Lichrospher-C18 (250 mm × 4. 6 mm, 5 μm) column with the mobile phase of methanol-acetic acid (24∶76). The detection wavelength was 232nm. Results:Paeoniae radix, Carthamus tinctorius, Coicis semen and Nutgrass galingale rhizome could be identified by TLC without any interference from the negative control. The linear range of peoniflorin was 6. 560-104. 920 μg·ml-1(r=0. 999 9) with the average recovery of 98. 77%(RSD=2. 73%,n=9). Conclusion:The qualitative identifi-cation is specific and reproducible, and the quantitative method is simple, accurate and reliable, which can be used in the quality con-trol of Pingyou granules.