CAJ | 학술논문

目的：：探讨清毒颗粒对二甲基苯蒽( DMBA)诱导SD大鼠乳腺癌的药效。方法：DMBA诱导大鼠乳腺癌,观察清毒颗粒对肿瘤抑制率,HE染色观察病理特点,IHC检测肿瘤组织Ki-67分布及含量,ELISA检测大鼠血清中IL-12、IFN-γ、IL-4和IL-10含量。结果：低、中、高剂量清毒颗粒和柴胡皂苷a的抑瘤率分别为：30.93%,43.84%,44.17%和43.48%；清毒颗粒和柴胡皂苷a可显著降低Ki-67表达量；上调血清中IL-12、IFN-γ含量,下调IL-4、IL-10含量。结论：清毒颗粒具有抗乳腺癌作用,其机制可能是调节机体免疫,增强抗肿瘤免疫作用。
목적：：탐토청독과립대이갑기분은( DMBA)유도SD대서유선암적약효。방법：DMBA유도대서유선암,관찰청독과립대종류억제솔,HE염색관찰병리특점,IHC검측종류조직Ki-67분포급함량,ELISA검측대서혈청중IL-12、IFN-γ、IL-4화IL-10함량。결과：저、중、고제량청독과립화시호조감a적억류솔분별위：30.93%,43.84%,44.17%화43.48%；청독과립화시호조감a가현저강저Ki-67표체량；상조혈청중IL-12、IFN-γ함량,하조IL-4、IL-10함량。결론：청독과립구유항유선암작용,기궤제가능시조절궤체면역,증강항종류면역작용。
To study the effect and mechanism of Qingdu granules on the tumor growth of 7, 12-dimethyl-benz[ a] an-thracene ( DMBA)-induced breast cancer in Sprague-Dawley rats. Methods: DMBA was used to induce breast cancer in rats. The tumor inhibition of Qingdu granules was observed. Pathological features were observed after hematoxylin-eosin staining, the distribution and content of Ki-67 in tumor were tested by IHC and the content of IL-12, IFN-γ, IL-4 and IL-10 in serum was determined by ELISA. Results:The inhibitory rate of Qingdu granules at low, middle and high dose and saikosaponin a was 30. 93%,43. 84% and 44. 17% and 43. 48%, respectively. The expression of Ki-67 was reduced in Qingdu granules groups and saikosaponin a group, the content of IL-12 and IFN-γ in serum was increased and the level of IL-4 and IL-10 was reduced in the above groups. Conclusion:Qingdu granules can inhibit breast cancer obviously, and the mechanism is probably related to the ability of immune system adjustment, which can enhance the antitumor effect.