CAJ | 학술논문

本研究旨在比较牛溶血性曼氏杆菌（M.haemolytica）和牛多杀性巴氏杆菌（P.multocida）A型、B型生理生化特性和PCR鉴定方法，为临床上述三种细菌的分离鉴定提供指导。培养牛溶血性曼氏杆菌、牛多杀性巴氏杆菌A型、B型菌，分别开展革兰氏染色镜检、生化鉴定、生长曲线测定、致病性检测和PCR鉴定。P.multocidaA型、B型血平板培养基上P.multocida不溶血、M.haemolytica溶血，在麦康凯培养基上P.multocida不生长、M.haemolytica生长良好；三株细菌在生化特性方面基本相同，但P.multocida能形成靛基质、不发酵肝糖和肌醇，M.haemolytica不能形成靛基质、可分解肝糖和肌醇。三株细菌生长趋势大致相同，均于2~4h进入稳定期，18~20h开始衰退。小鼠感染低至5个P.multocidaA型、B型菌可致死，M.haemolytica原液仍不能致死小鼠。所设计各菌株PCR引物能快速鉴定P.multocidaA型、B型和M.haemolytica。P.multocidaA型、B型和M.haemolytica在部分培养特性、生理生化特征、致病力及PCR鉴定方面存在显著差异。
본연구지재비교우용혈성만씨간균（M.haemolytica）화우다살성파씨간균（P.multocida）A형、B형생리생화특성화PCR감정방법，위림상상술삼충세균적분리감정제공지도。배양우용혈성만씨간균、우다살성파씨간균A형、B형균，분별개전혁란씨염색경검、생화감정、생장곡선측정、치병성검측화PCR감정。P.multocidaA형、B형혈평판배양기상P.multocida불용혈、M.haemolytica용혈，재맥강개배양기상P.multocida불생장、M.haemolytica생장량호；삼주세균재생화특성방면기본상동，단P.multocida능형성전기질、불발효간당화기순，M.haemolytica불능형성전기질、가분해간당화기순。삼주세균생장추세대치상동，균우2~4h진입은정기，18~20h개시쇠퇴。소서감염저지5개P.multocidaA형、B형균가치사，M.haemolytica원액잉불능치사소서。소설계각균주PCR인물능쾌속감정P.multocidaA형、B형화M.haemolytica。P.multocidaA형、B형화M.haemolytica재부분배양특성、생리생화특정、치병력급PCR감정방면존재현저차이。
In order to provide clinical guidance for isolation and identification for Mannheimia haemolytica, Pasteurella multocida type A and B strains, physiological and biochemical characteristics, PCR identiifcation method were researched in the study. After culturing Mannheimia haemolytica, Pasteurella multocida type A and B strains, gram staining, biochemical tests, growth curve, pathogenicity and PCR ampliifcation were valued respectively. There were no hemolysis of P.multocida type A and B strains on blood agar medium, but M. haemolytica was hemolysis. P. multocida did not grow on MacConkey medium, but M. haemolytica grew well. Three strains of bacteria were same in the most basic biochemical characteristics. P. multocida could form indole, but not fermented glycogen and inositol, whereas M. haemolytica was on the contrary. Three growth curves of strains were mostly same, which involved in the stable stage from 2 to 4 culturing hours and the decline stage from 18 to 20 culturing hours. Mice could be lethal with up to ifve P.multocida type A or B clones. Meanwhile, M. haemolytica could not be lethal with liquid bacterial culture. Three strains could be identiifed quickly and accurately using speciifc primers. There were significant differences in physiological and biochemical property, pathogenicity and PCR identification between Mannheimia haemolytica, Pasteurellamultocida type A and B strains.