CAJ | 학술논문

为建立一种牛传染性鼻气管炎病毒（IBRV）PCR检测方法，依据IBRV的tK基因序列，设计合成PCR的引物，以IBRV的标准AV20毒株为对象，确定PCR最终反应体系为25μL，并优化了PCR反应条件。结果显示，该PCR方法可以扩增出301bp的特异性片段；对牧场中常见的BVDV进行PCR特异性检测，未检测到BVDV特异性条带；10倍稀释法验证PCR优化反应后的灵敏性为可检测出0.002TCID50的病毒量。结果表明，该方法可为牧场进行IBR疫情监测提供有效技术手段。
위건립일충우전염성비기관염병독（IBRV）PCR검측방법，의거IBRV적tK기인서렬，설계합성PCR적인물，이IBRV적표준AV20독주위대상，학정PCR최종반응체계위25μL，병우화료PCR반응조건。결과현시，해PCR방법가이확증출301bp적특이성편단；대목장중상견적BVDV진행PCR특이성검측，미검측도BVDV특이성조대；10배희석법험증PCR우화반응후적령민성위가검측출0.002TCID50적병독량。결과표명，해방법가위목장진행IBR역정감측제공유효기술수단。
Bovineherpesvirus 1(BHV-1),the causative agent of infectious bovine rhinotracheitis(IBR), is considered to be the most common viral pathogen found in bovine. PCR ampliifcation was carried out in a ifnal volume of 25μL. The optimization of the PCR reaction components and cycling parameters was performed, respectively. The result of speciifc fragment for the standard IBRV by PCR ampliifed is 301bp. The analytical speciifcity of the PCR was determined by testing two different types of virus were BVDV and IBRV. The sensitivity of the PCR was determined by testing sequential 10-fold dilutions of IBRV. The establishment of PCR in the assay could be an useful tool for monitoring BHV-1 in the farm.