水产科学
水產科學
수산과학
FISHERIES SCIENCE
2015年
8期
497-501
,共5页
牟春艳%郑曙明%任胜杰%饶远%陈芃宇
牟春豔%鄭曙明%任勝傑%饒遠%陳芃宇
모춘염%정서명%임성걸%요원%진봉우
血鹦鹉鱼%色素细胞%类胡萝卜素
血鸚鵡魚%色素細胞%類鬍蘿蔔素
혈앵무어%색소세포%류호라복소
Amphilophus%chromatophore%carotenoid
用显微镜观察了体长7.0~10.0 cm的黑、黑黄、黄、红4种体色血鹦鹉鱼色素细胞的形态结构,用紫外分光光度计法测定了其总类胡萝卜素含量。结果显示,(1)黑色血鹦鹉鱼通体分布树突状和圆点状两种形态的黑色素细胞,内含大量黑色素颗粒,黄色素细胞较少,颜色较浅;(2)黑黄色血鹦鹉鱼体上的树突状黑色素细胞的中心胞体扩散,分枝细长,黑色素颗粒为棕色,聚集能力弱,黄色素细胞大颜色深且数量较多;(3)黄色血鹦鹉鱼体上的黑色素细胞数量很少,黄色素细胞遍体分布,颜色深,有少量圆点状的红色素细胞,形状小,颜色浅;(4)红色血鹦鹉鱼体的黑色素细胞稀少,有少量的黄色素细胞,红色素细胞遍体分布。(5)红色血鹦鹉鱼的肉、鳞片、鳍条和皮肤的类胡萝卜素含量与其他3种体色的鱼相比差异均极显著(P<0.01);黑、黑黄、黄3种体色的血鹦鹉鱼的上述4个部位的类胡萝卜素含量差异均不显著(P>0.05)。本试验结果为深入探究色素细胞的发育机制提供了参考。
用顯微鏡觀察瞭體長7.0~10.0 cm的黑、黑黃、黃、紅4種體色血鸚鵡魚色素細胞的形態結構,用紫外分光光度計法測定瞭其總類鬍蘿蔔素含量。結果顯示,(1)黑色血鸚鵡魚通體分佈樹突狀和圓點狀兩種形態的黑色素細胞,內含大量黑色素顆粒,黃色素細胞較少,顏色較淺;(2)黑黃色血鸚鵡魚體上的樹突狀黑色素細胞的中心胞體擴散,分枝細長,黑色素顆粒為棕色,聚集能力弱,黃色素細胞大顏色深且數量較多;(3)黃色血鸚鵡魚體上的黑色素細胞數量很少,黃色素細胞遍體分佈,顏色深,有少量圓點狀的紅色素細胞,形狀小,顏色淺;(4)紅色血鸚鵡魚體的黑色素細胞稀少,有少量的黃色素細胞,紅色素細胞遍體分佈。(5)紅色血鸚鵡魚的肉、鱗片、鰭條和皮膚的類鬍蘿蔔素含量與其他3種體色的魚相比差異均極顯著(P<0.01);黑、黑黃、黃3種體色的血鸚鵡魚的上述4箇部位的類鬍蘿蔔素含量差異均不顯著(P>0.05)。本試驗結果為深入探究色素細胞的髮育機製提供瞭參攷。
용현미경관찰료체장7.0~10.0 cm적흑、흑황、황、홍4충체색혈앵무어색소세포적형태결구,용자외분광광도계법측정료기총류호라복소함량。결과현시,(1)흑색혈앵무어통체분포수돌상화원점상량충형태적흑색소세포,내함대량흑색소과립,황색소세포교소,안색교천;(2)흑황색혈앵무어체상적수돌상흑색소세포적중심포체확산,분지세장,흑색소과립위종색,취집능력약,황색소세포대안색심차수량교다;(3)황색혈앵무어체상적흑색소세포수량흔소,황색소세포편체분포,안색심,유소량원점상적홍색소세포,형상소,안색천;(4)홍색혈앵무어체적흑색소세포희소,유소량적황색소세포,홍색소세포편체분포。(5)홍색혈앵무어적육、린편、기조화피부적류호라복소함량여기타3충체색적어상비차이균겁현저(P<0.01);흑、흑황、황3충체색적혈앵무어적상술4개부위적류호라복소함량차이균불현저(P>0.05)。본시험결과위심입탐구색소세포적발육궤제제공료삼고。
The morphology and number of chromatophores and carotenoids contents were observed in blood parrot with various body colors including black ,black‐yellow ,yellow ,and red colors under a microscope and measured by ultraviolet visible spectrophotometer .The results showed that the black blood parrots had many dendritic and dot shaped melanoma cells containing a lot of melanin granules ,and had a small amount of yellow pigment cells with light color on the whole body .The center cell body diffusion ,slender branches ,and weak aggregation in the dendritic melanocytes with brown black pigment granules were found in black yellow blood parrot with many large black yellow chromatophores .A large amount of yellow pigment cells was distributed on the whole body of black yellow blood parrot ,with a few melanocytes .The red pigment cells were covered on the red blood parrot body ,with scarce melanin cells and small amount of yellow pigment cells .There were significant differences in carotenoid contents in flesh ,scales ,fins and skins between red color blood parrots and black ,black‐yellow ,and yellow blood parrots (P<0 .01) ,without significant difference in carotenoid contents in the black ,black‐yellow ,and yellow blood parrots (P>0 .05) .The findings provides a theoretical basis with further study on mechanism of chromatophorogenesis .