CAJ | 학술논문

采用单因素试验方法研究富氮及限氮两种培养体系下甘油浓度（41、110、206 mmol／L ）对湛江等鞭金藻生长及细胞内多糖累积的影响。试验结果显示，当甘油浓度为41 mmol／L时湛江等鞭金藻的细胞密度、细胞内多糖含量及产量均比其他两种甘油浓度时的值高，其中富氮培养体系的细胞密度、细胞内多糖含量及产量分别为7．73×107个／m L、16．21％和0．32 g／L ，限氮培养体系的细胞密度、细胞内多糖含量及产量依次为5．29×107个／m L、54．43％和1．02 g／L。表明培养体系中甘油浓度低而氮含量高时有利于细胞生长（前者是后者的1．46倍），而甘油和氮含量均少时利于细胞积累多糖（后者的多糖含量和产量分别是前者的3．36倍和3．19倍）。在两种培养体系下，随着甘油浓度的增加，湛江等鞭金藻的细胞密度及藻细胞内累积的多糖呈降低趋势，说明高浓度甘油抑制湛江等鞭金藻细胞生长和细胞内多糖的累积。因此，为提高湛江等鞭金藻细胞内的多糖产量，应在接种时添加低浓度甘油的限氮培养体系中培养湛江等鞭金藻，这些结果可为高值天然产物的生产提供参考。
채용단인소시험방법연구부담급한담량충배양체계하감유농도（41、110、206 mmol／L ）대담강등편금조생장급세포내다당루적적영향。시험결과현시，당감유농도위41 mmol／L시담강등편금조적세포밀도、세포내다당함량급산량균비기타량충감유농도시적치고，기중부담배양체계적세포밀도、세포내다당함량급산량분별위7．73×107개／m L、16．21％화0．32 g／L ，한담배양체계적세포밀도、세포내다당함량급산량의차위5．29×107개／m L、54．43％화1．02 g／L。표명배양체계중감유농도저이담함량고시유리우세포생장（전자시후자적1．46배），이감유화담함량균소시리우세포적루다당（후자적다당함량화산량분별시전자적3．36배화3．19배）。재량충배양체계하，수착감유농도적증가，담강등편금조적세포밀도급조세포내루적적다당정강저추세，설명고농도감유억제담강등편금조세포생장화세포내다당적루적。인차，위제고담강등편금조세포내적다당산량，응재접충시첨가저농도감유적한담배양체계중배양담강등편금조，저사결과가위고치천연산물적생산제공삼고。
Effects of glycerol concentration (41 mmol/L ,110 mmol/L and 206 mmol/L ) on grow th and polysaccharides accumulation of alga Isochrysis zhanjiangensis were investigated in rich - nitrogen and limited-nitrogen culture systems by means of single factor experiments .The results showed that the cell density ,content and yield of polysaccharides were higher in 41mmol/L glycerol than those in the other glycerol concentrations . T he cell density , content and yield of polysaccharides in rich‐nitrogen culture system were 7 .73 × 107 cells/mL ,16 .21% and 0 .32 g/L ,respectively ,while those in limited‐nitrogen culture system were 5 .29 × 107 cells/mL ,54 .43% and 1 .02 g/L ,respectively .These data explained that lower glycerol concentration and higher nitrogen concentration were advantageous to the growth of I . zhanjiangensis (the biomass in the former was 1 .46 times as that in the later) ,however ,when glycerol concentration and nitrogen concentration were lower at the same time , the cell accumulated more polysaccharides (the content and the yield of polysaccharides of the later were 3 .36 times and 3 .19 times as the former ,respectively ) .No matter the difference in nitrogen supply ,with the increasing concentration of glycerol ,the cell density and polysaccharides content of I .zhanjiang‐ensis showed a decreasing trend . It indicated that high concentration of glycerol inhibited the growth of I .zhangjiangensis and the accumulation of polysaccharides .So ,I .zhangjiangensis should be cultured in the limited‐nitrogen culture system with low concentration of glycerol at inoculation to promote the accumulation of polysaccharides . The findings will provide reference with producing natural products of high value .