CAJ | 학술논문

以脱脂粉为原料，利用酶解法提取制备核桃多肽。在单因素试验的基础上，根据Box-Behnken的中心组合实验设计原理，运用Design Expert8.0.6数据统计分析软件，采用四因素三水平的响应面分析法，以多肽得率为响应值，研究了底物质量浓度、温度、时间、pH对核桃多肽得率的影响，并优化了提取工艺。确定了酶解法提取核桃多肽的最佳工艺条件为：底物质量浓度2.3%，温度51℃，时间5.2 h，pH6.5，在此条件下多肽得率为0.45 g/g。
이탈지분위원료，이용매해법제취제비핵도다태。재단인소시험적기출상，근거Box-Behnken적중심조합실험설계원리，운용Design Expert8.0.6수거통계분석연건，채용사인소삼수평적향응면분석법，이다태득솔위향응치，연구료저물질량농도、온도、시간、pH대핵도다태득솔적영향，병우화료제취공예。학정료매해법제취핵도다태적최가공예조건위：저물질량농도2.3%，온도51℃，시간5.2 h，pH6.5，재차조건하다태득솔위0.45 g/g。
Walnut polypeptide was obstained by enzymatic hydrolysis with the defatted walnut meal as raw material.The preparation process of walnut polypeptide was optimized on foundation of single factor experiment , according to Box-Behnken central composition design principle ,the method of response surface analysis with 4 factors and 3 levels combined with Design Expert8.0.6-statistical data analysis software was adopted with the leaching rate of walnut polypeptide as the response value. The effect of substrate concentration, temperature, time and pH on the leaching rate of walnut polypeptide were studied in this paper. According to the above results, the optimum preparation conditions were obtained as follows: substrate concentration 2.3 % , temperature 51 ℃,time 5.2 h, and pH 6.5.Under these conditions, the leaching rate of walnut polypeptide reached 0.45 g/g.