黑龙江科技信息
黑龍江科技信息
흑룡강과기신식
HEILONGJIANG SCIENCE AND TECHNOLOGY INFORMATION
2015年
26期
10-10
,共1页
复方银杏叶口服液%槲皮素%高效液相色谱法
複方銀杏葉口服液%槲皮素%高效液相色譜法
복방은행협구복액%곡피소%고효액상색보법
Compound ginkgo leaf oral liqiud%Quercetin%HPLC
目的：建立复方银杏叶口服液中槲皮素的含量测定方法。方法：高效液相色谱法，采用C18（250×4.6mm，5μm）色谱柱；甲醇-0.4%磷酸溶液（50：50）为流动相；流速1.0mL·min-1；检测波长360nm。结果：在4.81440μg·mL-1～60.18000μg·mL-1浓度范围内槲皮素的峰面积和浓度具有良好的线性关系，R=0.99992；平均回收率为98.77%，RSD=0.84%（n=9）。结论：本方法准确、可靠、重复性好，适用于复方银杏叶口服液的质量控制。
目的：建立複方銀杏葉口服液中槲皮素的含量測定方法。方法：高效液相色譜法，採用C18（250×4.6mm，5μm）色譜柱；甲醇-0.4%燐痠溶液（50：50）為流動相；流速1.0mL·min-1；檢測波長360nm。結果：在4.81440μg·mL-1～60.18000μg·mL-1濃度範圍內槲皮素的峰麵積和濃度具有良好的線性關繫，R=0.99992；平均迴收率為98.77%，RSD=0.84%（n=9）。結論：本方法準確、可靠、重複性好，適用于複方銀杏葉口服液的質量控製。
목적：건립복방은행협구복액중곡피소적함량측정방법。방법：고효액상색보법，채용C18（250×4.6mm，5μm）색보주；갑순-0.4%린산용액（50：50）위류동상；류속1.0mL·min-1；검측파장360nm。결과：재4.81440μg·mL-1～60.18000μg·mL-1농도범위내곡피소적봉면적화농도구유량호적선성관계，R=0.99992；평균회수솔위98.77%，RSD=0.84%（n=9）。결론：본방법준학、가고、중복성호，괄용우복방은행협구복액적질량공제。
Objective:To establish an assay of determination of Quercetin in Compound ginkgo leaf oral liqiud by HPLC.Method:HPLC was carried out on a C18 (250 ×4.6mm,5μm) column;The mobile phase consisted of methanol –0.4% phosphoric acid solutions (50:50) with the flow rate of 1.0 mL?min-1.The detection wave length was 360nm.Results:The standard curve was in good linearity in the range of 4.81440μg·mL-1~60.18000μg·mL-1,R=0.99992 for determination of its related substances.The average recovery rate was 98.77% with RSD of 0.84%.Conclusion:The method is accurate,reliable and reproducible .The method can be used for the quality control of Compound ginkgo leaf oral liqiud.
