CAJ | 학술논문

柞蚕受螽斯危害严重，利用商品化颗粒饵剂防治害蚕螽斯是防治方法的革新。建立了在同一液相色谱条件下测定防治害蚕螽斯饵剂中毒死蜱含量的方法。本方法采用 NH2基 SPE 固相萃取柱滤过不易分离的凡士林、蜂蜡杂质，用 C18色谱柱，以乙腈—水—冰乙酸（体积比82：17.5：0.5）为流动相，在检测波长290 nm下，外标法对饵剂中毒死蜱进行定量分析。分析结果表明，标准偏差为0.0075，变异系数为1.45%；平均回收率为97.87%；线性相关系数相关系数 r =0.9995，是对在线产品检测的理想方法。
작잠수종사위해엄중，이용상품화과립이제방치해잠종사시방치방법적혁신。건립료재동일액상색보조건하측정방치해잠종사이제중독사비함량적방법。본방법채용 NH2기 SPE 고상췌취주려과불역분리적범사림、봉사잡질，용 C18색보주，이을정—수—빙을산（체적비82：17.5：0.5）위류동상，재검측파장290 nm하，외표법대이제중독사비진행정량분석。분석결과표명，표준편차위0.0075，변이계수위1.45%；평균회수솔위97.87%；선성상관계수상관계수 r =0.9995，시대재선산품검측적이상방법。
Katydids caused serious damage to rear of antherae perny. The control of Katydids via tablet bait is the innovation of control method. An HPLC method was developed for the determination of chlorpyrifos in bait for killing Katydids. The method use NH2 based SPE column filtration to remove Vaseline and beeswax impu-rities,which is not easy to separate conventionally. The HPLC conditions were via C18 column using acetoni-trile-water-glacial acetic acid = 82: 17. 5: 0. 5( by vol)as mobile phase with UV detection at 290 nm. The results showed that the variation coefficients,standard deviations and average recoveries of chlorpyrifos were 0. 0075,1. 45% ,97. 87% ,respectively,with a linear correlations of 0. 9995.