辽宁农业科学
遼寧農業科學
료녕농업과학
LIAONING AGRICULTURAL SCIENCES
2015年
4期
1-7
,共7页
吴禹%陈爱国%王岩%李兆波%路明祥%孟未来%崔晓光
吳禹%陳愛國%王巖%李兆波%路明祥%孟未來%崔曉光
오우%진애국%왕암%리조파%로명상%맹미래%최효광
野生大豆%遗传多样性%遗传相似性%聚类分析%SSR
野生大豆%遺傳多樣性%遺傳相似性%聚類分析%SSR
야생대두%유전다양성%유전상사성%취류분석%SSR
Wild soybean%Genetic diversity%Genetic similarity%Cluster analysis%SSR
2013年对辽宁省铁岭市、大连市、朝阳市的野生大豆进行了初步考察、收集和整理。利用20对 SSR 引物对来自辽宁省3个地级市的120份野生大豆种质进行了遗传多态性分析。结果表明,辽宁省野生大豆具有较高的遗传多样性,20对 SSR 引物全部在野生大豆中扩增出多态产物,多态性位点所占比例为100%,Shan-non 多样性指数平均为1.6517。野生大豆群体的基因分化系数( Gst)平均为0.1022,野生大豆群体内的遗传分化较大,遗传差异明显。聚类分析中,3个地区共分为2个大类和12个小类,表明材料的遗传背景与地理分布具有一定的相关性。
2013年對遼寧省鐵嶺市、大連市、朝暘市的野生大豆進行瞭初步攷察、收集和整理。利用20對 SSR 引物對來自遼寧省3箇地級市的120份野生大豆種質進行瞭遺傳多態性分析。結果錶明,遼寧省野生大豆具有較高的遺傳多樣性,20對 SSR 引物全部在野生大豆中擴增齣多態產物,多態性位點所佔比例為100%,Shan-non 多樣性指數平均為1.6517。野生大豆群體的基因分化繫數( Gst)平均為0.1022,野生大豆群體內的遺傳分化較大,遺傳差異明顯。聚類分析中,3箇地區共分為2箇大類和12箇小類,錶明材料的遺傳揹景與地理分佈具有一定的相關性。
2013년대요녕성철령시、대련시、조양시적야생대두진행료초보고찰、수집화정리。이용20대 SSR 인물대래자요녕성3개지급시적120빈야생대두충질진행료유전다태성분석。결과표명,요녕성야생대두구유교고적유전다양성,20대 SSR 인물전부재야생대두중확증출다태산물,다태성위점소점비례위100%,Shan-non 다양성지수평균위1.6517。야생대두군체적기인분화계수( Gst)평균위0.1022,야생대두군체내적유전분화교대,유전차이명현。취류분석중,3개지구공분위2개대류화12개소류,표명재료적유전배경여지리분포구유일정적상관성。
Field surveys were conducted to define the distribution region of Liaoning wild soybeans,wild soy-beans resources were collected from three regions of Liaoning Province in the autumns 2013. The genetic di-versity was analyzed to 120 wild soybean germplasms from 3 regions in Liaoning province by using 20 pairs of SSR primers. The percentage of polymorphic loci was 100% and Shannonˊs information index was 1. 6517, showing that the genetic diversity of Liaoning wild soybean is very high. The Gst of 0. 1022 suggested that the main genetic variation of wild soybean resided internal populations. The 3 areas were divided into 2 major cat-egories and 12 sub-categories in clustering analysis,which showed that the genetic background of germplasm was correlated to its geographical distribution to some extent.