CAJ | 학술논문

为了获得对新疆苹果树腐烂病(Cytospora spp．)有较好拮抗作用的生防菌株.采用组织分离法和平板对峙法筛选得到拮抗效果较好的菌株.通过形态学、生理生化特性及分子生物学方法确定生防菌株的分类地位.最终筛选得到对病菌拮抗效果达到87％的拮抗菌株 xj019.菌株 xj019的最佳培养时间为24 h,最适 pH 值为7.结合形态学特征、生理生化特征和16S rDNA 序列分析将菌株 xj019鉴定为枯草芽孢杆菌(Bacillus subtilis ).所获得的拮抗菌株 xj019对苹果树腐烂病菌的生物防治具有潜在的应用价值.
위료획득대신강평과수부란병(Cytospora spp．)유교호길항작용적생방균주.채용조직분리법화평판대치법사선득도길항효과교호적균주.통과형태학、생리생화특성급분자생물학방법학정생방균주적분류지위.최종사선득도대병균길항효과체도87％적길항균주 xj019.균주 xj019적최가배양시간위24 h,최괄 pH 치위7.결합형태학특정、생리생화특정화16S rDNA 서렬분석장균주 xj019감정위고초아포간균(Bacillus subtilis ).소획득적길항균주 xj019대평과수부란병균적생물방치구유잠재적응용개치.
In order to obtain biological control strains,which have better antagnictic functions for Cyto-spora spp.in Xinjiang,the tissure isolation and flat controntation culture method were used to screen the biocontrol agents.The biocontrol agents were indentified according to the morphological and phisiological characteristics and molecular and biological method.Finally antagonitic bacterials strain xj01 9 were found. The eptimum incubation time was 24 h for strains,optimum pH was 7.The strain xj01 9 was identified as Bacillus subtilis .The antagonistic strains xj019 obtained were of great value in biological control of apple canker.