CAJ | 학술논문

为了探讨新疆野杏种质资源的遗传多样性和亲缘关系,利用 ISSR 分子标记技术,对新疆伊犁地区3个居群、135份野杏种质资源进行了 PCR 扩增.研究表明,22条引物共扩增出465条多态性条带,多态性位点百分率为99．49％；从物种水平分析,新疆野杏种质资源的多态性位点百分率、观测等位基因数、有效等位基因数、Nei’s 基因多样性和 Shannon 信息指数分别为99．57％,1．9957,1．3879,0．2449,0．3874；从居群水平分析,各居群的多态性位点百分率、观测等位基因数、有效等位基因数、Nei’s 基因多样性和 Shannon 信息指数各指标的平均值分别为76．80％,1．7680,1．3463,0．2120和0．3287；3个居群的各项指标均为霍城居群最高,新源居群最低,巩留居群居中；新疆野杏种质资源的遗传变异主要来自于居群内(86．43％),少部分来自于居群间(13．57％)；各居群中霍城居群和新源居群亲缘关系最近,遗传分化程度小；新源居群和巩留居群间亲缘关系最远,遗传分化程度大.
위료탐토신강야행충질자원적유전다양성화친연관계,이용 ISSR 분자표기기술,대신강이리지구3개거군、135빈야행충질자원진행료 PCR 확증.연구표명,22조인물공확증출465조다태성조대,다태성위점백분솔위99．49％；종물충수평분석,신강야행충질자원적다태성위점백분솔、관측등위기인수、유효등위기인수、Nei’s 기인다양성화 Shannon 신식지수분별위99．57％,1．9957,1．3879,0．2449,0．3874；종거군수평분석,각거군적다태성위점백분솔、관측등위기인수、유효등위기인수、Nei’s 기인다양성화 Shannon 신식지수각지표적평균치분별위76．80％,1．7680,1．3463,0．2120화0．3287；3개거군적각항지표균위곽성거군최고,신원거군최저,공류거군거중；신강야행충질자원적유전변이주요래자우거군내(86．43％),소부분래자우거군간(13．57％)；각거군중곽성거군화신원거군친연관계최근,유전분화정도소；신원거군화공류거군간친연관계최원,유전분화정도대.
ISSR molecular marker technology was used to amplify 3 populations of wild apricot resources in Yili area,Xinjiang,135 wild apricot samples in order to discuss genetic diversity and genetic relationships of wild apricot germplasm resources in Xinjiang.The results showed that 465 polymorphism bands were amplified with 22 primers by the ISSR-PCR amplification,the percentage of polymorphism loci was 99.49%.Analysis from the species level showed that percentage of polymorphic loci,observed number of al-leles,effective number of alleles,Nei’s genetic diversity and Shannon information index were 99.57%, 1.9957,1.3879,0.2449 and 0.3874 respectively;Analysis from the population levels showed that the aver-age values of percentage of the polymorphic loci,observed number of alleles,effective number of alleles, Nei’s genetic diversity and Shannon information index were 76.80%,1.7680,1.3463,0.2120 and 0.3287 re-spectively.Huocheng ranks first among the different index of three populations,Xinyuan population got the minimum,Gongliu population was in the middle.The genetic variation of the wild apricot resources in Xin-jiang mainly existed in the intra-population (84.85%),and a few existed in the inter-population (15.15%). The genetic relationship of Houcheng population and Xinyuan population was the closest,the genetic varia-tion degree was the smallest;But the genetic relationship between Xinyuan population and Gongliu popula-tion was the farthest,the genetic variation degree was greatest.