CAJ | 학술논문

为了研究 MADS-box A 类基因在莲花器官发育中的作用，以莲品种‘大洒锦’（Nelumbo nucifera ‘Da Sajin’）的花蕾为试验材料，利用 rT-PCr 方法克隆了莲 FUL-like 基因 cDNA 序列，命名为 NnFUL。NnFUL 的cDNA长度为763 bp，其开放阅读框（Open reading frame，OrF）共编码250个氨基酸。该蛋白属于亲水性蛋白，二级结构主要由α-螺旋、无规则卷曲和延伸链组成。系统进化分析表明：NnFUL 编码的蛋白与 AP1？SQUA 亚家族中的FUL-like 蛋白聚为一类，与二球悬铃木（Platanus ×acerifolia ）的 FUL-like 蛋白具有较高的亲缘关系。亚细胞定位显示该基因位于细胞核中。荧光定量 real-time PCr 结果表明：NnFUL 在花器官中表达量最高，主要集中在花萼和花瓣中，同时在营养器官中也有表达；因此，NnFUL 在花器官发育尤其花萼和花瓣形成中有一定的调控作用。
위료연구 MADS-box A 류기인재연화기관발육중적작용，이련품충‘대쇄금’（Nelumbo nucifera ‘Da Sajin’）적화뢰위시험재료，이용 rT-PCr 방법극륭료련 FUL-like 기인 cDNA 서렬，명명위 NnFUL。NnFUL 적cDNA장도위763 bp，기개방열독광（Open reading frame，OrF）공편마250개안기산。해단백속우친수성단백，이급결구주요유α-라선、무규칙권곡화연신련조성。계통진화분석표명：NnFUL 편마적단백여 AP1？SQUA 아가족중적FUL-like 단백취위일류，여이구현령목（Platanus ×acerifolia ）적 FUL-like 단백구유교고적친연관계。아세포정위현시해기인위우세포핵중。형광정량 real-time PCr 결과표명：NnFUL 재화기관중표체량최고，주요집중재화악화화판중，동시재영양기관중야유표체；인차，NnFUL 재화기관발육우기화악화화판형성중유일정적조공작용。
To investigate the effect of FUL-like gene of A-class MADS-box family on floral organ develop-ment of Nelumbo nucifera,flower buds of N.nucifera ‘Da Sajin’were used as a test material and a cDNA of a FUL-like gene,being named NnFUL and 763 bp long,was successfully cloned by rT-PCr.The cDNA’s open reading frame (OrF)encodes 250 amino acids totally.The NnFUL protein is hydrophilic and its secondary struc-ture mainly consists of α-helical,irregularly curled and extended strands.The phylogenetic analysis showed that the NnFUL protein belongs to a class of FUL-like protein in AP1 ?SQUA subfamily and has a close genetic rela-tionship with FUL-like protein of Platanus ×acerifolia.The subcellular localization indicated that the gene NnFUL is located in the cell nucleus.The fluorescent quantitation real-time PCr analysis showed that NnFUL has the highest expression level in the floral organ,mainly concentrating in the sepals and petals,and also has some expression in the vegetative organs.Therefore,the gene NnFUL has a certain regulatory effect on development of both sepals and petals in lotus flowers.