CAJ | 학술논문

从上海及周边地区的香菇子实体和培养料中分离污染真菌，并进行形态学观察和分子生物学鉴定。提取菌株基因组 DNA，利用真菌通用引物 ITS1和 ITS4扩增菌株 rDNA ITS 区序列，测序后在数据库中进行比对分析，并利用软件 MEGA5构建菌株分子系统发育树。结果表明：香菇栽培到储藏过程中主要污染真菌有木霉属（Trichoderma）、曲霉属（Aspergillus）、附球菌属（Epicoccum）、镰孢属（Fusarium）、青霉属（Penicillium）和节菱孢属（Arthrinium）等。
종상해급주변지구적향고자실체화배양료중분리오염진균，병진행형태학관찰화분자생물학감정。제취균주기인조 DNA，이용진균통용인물 ITS1화 ITS4확증균주 rDNA ITS 구서렬，측서후재수거고중진행비대분석，병이용연건 MEGA5구건균주분자계통발육수。결과표명：향고재배도저장과정중주요오염진균유목매속（Trichoderma）、곡매속（Aspergillus）、부구균속（Epicoccum）、렴포속（Fusarium）、청매속（Penicillium）화절릉포속（Arthrinium）등。
Symbiotic fungus from fruit body and substrate of Lentinula edodes in and around Shanghai were separated for morphological observation and molecular identification.By determining the sequence of a ribosomal DNA internal transcribed spacer (ITS),and comparing with corresponding sequences deposited in NCBI based on the closest match of BLAST analysis,the strains molecular phylogenetic tree was built using MEGA5.The re-sults showed that the main fungus contaminated during the process of cultivation to storage were Arthrinium,Epic-occum,Fusarium,Penicillium,Trichoderma and Aspergillus.