CAJ | 학술논문

建立了鸡蛋中游离棉酚和总棉酚的高效液相色谱（HPLC）检测方法。游离棉酚采用 L-抗坏血酸丙酮溶液提取，总棉酚经草酸丁酮水溶液水解后测定。采用 Waters XBridgeTM C18柱（250 mm ×4.6 mm，5μm），以乙腈-0.2％磷酸水溶液（v/v：85/15）为流动相，流速为1.0 mL/min，检测波长235 nm。该方法在0.1—50.0μg/mL范围内线性关系良好（r2≥0.9998），添加回收率在89.6％—91.7％，相对标准偏差为1.10％—4.06％（n ＝6），检出限为0.2 mg/kg，定量限为0.5 mg/kg。该方法简单、准确、灵敏度高，适用于鸡蛋中棉酚的检测。
건립료계단중유리면분화총면분적고효액상색보（HPLC）검측방법。유리면분채용 L-항배혈산병동용액제취，총면분경초산정동수용액수해후측정。채용 Waters XBridgeTM C18주（250 mm ×4.6 mm，5μm），이을정-0.2％린산수용액（v/v：85/15）위류동상，류속위1.0 mL/min，검측파장235 nm。해방법재0.1—50.0μg/mL범위내선성관계량호（r2≥0.9998），첨가회수솔재89.6％—91.7％，상대표준편차위1.10％—4.06％（n ＝6），검출한위0.2 mg/kg，정량한위0.5 mg/kg。해방법간단、준학、령민도고，괄용우계단중면분적검측。
A method to determine gossypol in egg was established by high performance liquid chromatogra-phy (HPLC).The free gossypol was extracted with L-ascorbic acid and acetone solution and the total gossypol was determined after being hydrolyzed by oxalic acid and butanone solution.The sample was separated on a C18 column (250 mm ×4.6 mm,5 μm),acetonitrile-0.2% phosphoric acid solution (v/v:85/15)was used as a mo-bile phase,the flow rate was 1.0 mL/min and the detection wavelength 235 nm.The results showed that there was a good linear relationship within 0.1—50.0 μg/mL (r2 ≥0.9998),the recovery rate and the relative standard deviation (rSD)were respectively 89.6%—91.7% and 1.10%—4.06% (n =6),and the detection limit and the quantitation limit were 0.2 mg/kg and 0.5 mg/kg respectively.This method was simple,sensitive and accu-rate,being suitable for determination of gossypol in egg.