CAJ | 학술논문

研究了佐剂蛋白大肠杆菌热敏性肠毒素 LTrG 与鸡新城疫疫苗（NDV）联合冻干制品的免疫效果。选择不同剂量的佐剂蛋白与鸡新城疫病毒（Newcastle disease virus，NDV）LaSota 抗原配比，按生产程序制备冻干疫苗。经滴鼻途径免疫雏鸡，检测血清抗体、粘膜抗体水平，分析不同配伍疫苗的免疫效果。结果表明：含4μg LTrG 佐剂蛋白的 NDV 冻干疫苗，免疫雏鸡血清 IgG 抗体水平明显高于其他添加组及 NDV 疫苗组，差异显著；粘膜 IgA 抗体与 NDV 疫苗组比较，差异极显著。LTrG 佐剂蛋白与鸡新城疫疫苗配伍制备冻干疫苗，能增强免疫雏鸡的免疫应答能力，尤其是可产生较好的粘膜抗体水平。
연구료좌제단백대장간균열민성장독소 LTrG 여계신성역역묘（NDV）연합동간제품적면역효과。선택불동제량적좌제단백여계신성역병독（Newcastle disease virus，NDV）LaSota 항원배비，안생산정서제비동간역묘。경적비도경면역추계，검측혈청항체、점막항체수평，분석불동배오역묘적면역효과。결과표명：함4μg LTrG 좌제단백적 NDV 동간역묘，면역추계혈청 IgG 항체수평명현고우기타첨가조급 NDV 역묘조，차이현저；점막 IgA 항체여 NDV 역묘조비교，차이겁현저。LTrG 좌제단백여계신성역역묘배오제비동간역묘，능증강면역추계적면역응답능력，우기시가산생교호적점막항체수평。
Necastle disease virus (NDV)combined with adjuvant protein—heat labile enterotoxin LTrG Escherichia coli were frozen into dried vaccine and the immune effect of the vaccine was studied in chicken.Dif-ferent dose of LTrG protein was mixed with NDV LaSota virus antigen,and the freeze-dried vaccine was made according to production process.The chicken were immunized by nasal vaccination.The serum IgG and mucosal IgA antibody were detected by ELISA method to evaluate the immune effect of different compatibility vaccine. The results showed that serum IgG antibody levels of NDV vaccine with 4 μg LTrG protein were significantly higher than those in other groups and NDV vaccine group,and mucosal IgA antibody levels were very significant-ly higher than those in NDV vaccine group.The freeze-dried vaccine which was adjuvant protein of LTrG com-bined with Newcastle disease vaccine could enhance the immune response ability of chicken,especially produce better mucosal antibody level.