CAJ | 학술논문

为探讨复合酸对老龄蛋鸡日粮pH值及消化道内环境的影响,将864只43周龄海兰灰种鸡随机分为Ⅰ组(基础日粮)、Ⅱ组(基础日粮+0.2%柠檬酸)和Ⅲ组(基础日粮+0.2%复合酸),测定试验鸡日粮pH值、酸结合力,试验第1周末( T1)及第5周末( T2)测试验鸡消化道pH值、小肠消化酶活性和盲肠菌群。结果表明：与Ⅰ组相比,Ⅱ组、Ⅲ组日粮pH值、酸结合力均显著降低。Ⅲ组嗉囊、腺胃pH值T1时期极显著低于Ⅱ组、Ⅰ组,T2时期显著低于Ⅱ组、Ⅰ组。Ⅲ组肌胃pH值T1时期分别较Ⅰ组、Ⅱ组降低9.72%(P<0.01)和4.06%(P<0.05),T2时期分别较Ⅰ组、Ⅱ组降低8.25%(P<0.05)和0.37%(P>0.05)。与Ⅰ组相比,T1时期,Ⅱ组、Ⅲ组淀粉酶活性分别提高10.16%(P <0.05)和16.61%(P <0.05),T2时期分别提高10.22%(P <0.05)和18.41%(P<0.05)。 T2时期,与Ⅰ组相比,Ⅱ组、Ⅲ组乳酸杆菌分别提高7.21%(P <0.05)和8.24%(P<0.05),大肠杆菌分别降低7.92%(P<0.05)和15.45%(P<0.01)。表明,酸化剂可显著降低日粮pH值和酸结合力,改善老龄蛋鸡消化道内环境,且复合酸的效果优于柠檬酸。
위탐토복합산대노령단계일량pH치급소화도내배경적영향,장864지43주령해란회충계수궤분위Ⅰ조(기출일량)、Ⅱ조(기출일량+0.2%저몽산)화Ⅲ조(기출일량+0.2%복합산),측정시험계일량pH치、산결합력,시험제1주말( T1)급제5주말( T2)측시험계소화도pH치、소장소화매활성화맹장균군。결과표명：여Ⅰ조상비,Ⅱ조、Ⅲ조일량pH치、산결합력균현저강저。Ⅲ조소낭、선위pH치T1시기겁현저저우Ⅱ조、Ⅰ조,T2시기현저저우Ⅱ조、Ⅰ조。Ⅲ조기위pH치T1시기분별교Ⅰ조、Ⅱ조강저9.72%(P<0.01)화4.06%(P<0.05),T2시기분별교Ⅰ조、Ⅱ조강저8.25%(P<0.05)화0.37%(P>0.05)。여Ⅰ조상비,T1시기,Ⅱ조、Ⅲ조정분매활성분별제고10.16%(P <0.05)화16.61%(P <0.05),T2시기분별제고10.22%(P <0.05)화18.41%(P<0.05)。 T2시기,여Ⅰ조상비,Ⅱ조、Ⅲ조유산간균분별제고7.21%(P <0.05)화8.24%(P<0.05),대장간균분별강저7.92%(P<0.05)화15.45%(P<0.01)。표명,산화제가현저강저일량pH치화산결합력,개선노령단계소화도내배경,차복합산적효과우우저몽산。
The effects of compound acid on dietary pH and gastrointestinal environments in old laying hens were studied by feeding trials with compound acid. 864 Hy-line gray breeding layers of 43 weeks were randomly divided into groupⅠ(basal diet),groupⅡ(basal diet+0. 2% citric acid)and group Ⅲ(basal diet+0. 2% compound acid). The pH and acid binding force of diet were determined before feed-ing. Gastrointestinal pH and the activity of digestive enzyme in small intestine and the flora in cecum from test chickens were determined at the first weekend(T1) and the fifth weekend(T2) during test. The re-sults indicated that the dietary pH and acid binding force in groupⅡand Ⅲ were significantly reduced ( P<0 . 05 ) than groupⅠ. The pH of craw and gland stomach from chickens in groupⅢwere very signifi-cantly reduced during T1(P<0. 01) and significantly reduced during T2(P<0. 05) than groupⅡ,and the pH of muscular stomach from chickens in group Ⅲ decreased by 9 . 72%( P <0 . 01 ) and 4 . 06%( P<0 . 05 ) during T1 and reduced 8 . 25%( P<0 . 05 ) and 0 . 37%( P>0 . 05 ) during T2 than groupⅠandⅡ,respectively. The activity of amylase in small intestine of chichens in groupⅡandⅢincreased by 10 . 16%( P<0 . 05 ) and 16 . 61%( P <0 . 05 ) during T1 and increased by 10 . 22%( P <0 . 05 ) and 18 . 41%( P<0 . 05 ) during T2 than groupⅠ. The number of lactobacillus in the cecum of chickens in groupⅡandⅢincreased by 7. 21%(P<0. 05) and 8. 24%(P<0. 05) than groupⅠduring T2,and the number of E. coli in the cecum of chichens in groupⅡand Ⅲ decreased by 7. 92%( P <0. 05 ) and 15. 45%(P < 0. 01) than groupⅠduring T2, respectively. The results showed that acidulant could obviousely reduce the pH and acid binding force of diet and improve gastrointestinal environments in old laying hens,and the effect of compound acid was superior to the citric acid.