CAJ | 학술논문

目的：：研究不同温度下可卡因与牛血清白蛋白(BSA)之间的相互作用及其作用机制。方法：通过荧光光谱法研究可卡因对BSA的荧光猝灭光谱和同步荧光光谱，确定荧光猝灭方式，并根据热力学方程讨论两者间的作用力类型。结果：可卡因对BSA的荧光呈规律性猝灭，分别用Stern-Volmer方程、Lineweaver-Burk方程和热力学方程等分析处理实验数据，求得在不同温度下反应的结合常数K分别为1.38×103 L/mol (30℃)和6.19×103 L/mol (37℃)、结合位点数n为1、热力学参数ΔH、ΔS、ΔG分别为167.44 KJ/mol、612.71J/mol·K、-18.21 KJ/mol(30℃)和-22.50 KJ/mol(37℃)。结论：可卡因对BSA的荧光猝灭机制为静态猝灭。两者的相互作用力主要是疏水作用力。
목적：：연구불동온도하가잡인여우혈청백단백(BSA)지간적상호작용급기작용궤제。방법：통과형광광보법연구가잡인대BSA적형광졸멸광보화동보형광광보，학정형광졸멸방식，병근거열역학방정토론량자간적작용력류형。결과：가잡인대BSA적형광정규률성졸멸，분별용Stern-Volmer방정、Lineweaver-Burk방정화열역학방정등분석처리실험수거，구득재불동온도하반응적결합상수K분별위1.38×103 L/mol (30℃)화6.19×103 L/mol (37℃)、결합위점수n위1、열역학삼수ΔH、ΔS、ΔG분별위167.44 KJ/mol、612.71J/mol·K、-18.21 KJ/mol(30℃)화-22.50 KJ/mol(37℃)。결론：가잡인대BSA적형광졸멸궤제위정태졸멸。량자적상호작용력주요시소수작용력。
Objective To study the interaction of bovine serum albumin(BSA) with cocaine at different temperature and mechanisms underlying. Methods The quenching of fluorescence was determined by measuring fluorescence quenching spectra and synchronous fluorescence spectra of BSA interacted with cocaine.The type of binding foece was estimated according to the themodynamic equation.Results The fluorescence of BSA interacted with cocaine was quenched regularly .The values of binding constants(Ks)to be 1.38×103L/mol (30℃),and6.19×103 L/mol (37℃),amounts of binding sites(1),and themodynamic parameters(ΔH: 167.44 KJ/mol,ΔS: 612.71J/mol·K ,ΔG: -18.21 KJ/mol(30℃)and-22.50(37℃))were obtained by analyzing and processing the fluorescence quenching data according to Stern-Volmer equation, Lineweaver-Burk equation and themodynamic equation,respectively.Conclusion It was shown that the fluorescence quenching process of BSA with Cocaine was attributed to static quenching. The force of the reaction was mainly due to hydrophobic action.