实验技术与管理
實驗技術與管理
실험기술여관리
EXPERIMENTAL TECHNOLOGY AND MANAGEMENT
2015年
8期
58-61
,共4页
吴子安%谭志容%徐宁%李涛%李曼%尹芳芳
吳子安%譚誌容%徐寧%李濤%李曼%尹芳芳
오자안%담지용%서저%리도%리만%윤방방
hepG2细胞%人斯钙素-1%人斯钙素-2%缺氧%复氧
hepG2細胞%人斯鈣素-1%人斯鈣素-2%缺氧%複氧
hepG2세포%인사개소-1%인사개소-2%결양%복양
hepG2 cell%STC-1%STC-2%anoxia%reoxygen
目的：研究缺氧及复氧对hepG2细胞STC‐1和STC‐2的mRNA表达影响。方法：用化学缺氧剂氯化钴模拟缺氧环境，用RT‐PCR法分别检测在hepG2细胞缺氧和复氧条件下STC‐1、STC‐2的mRNA表达情况。结果：在hepG2细胞中，STC‐1 mRNA无论在缺氧还是复氧情况下，几乎不表达；STC‐2 mRNA在缺氧条件下表达升高，并随着缺氧时间延长而升高，复氧后表达又降低，并随复氧时间延长而恢复至正常。结论：STC‐2与肝癌的发生发展过程密切相关。
目的：研究缺氧及複氧對hepG2細胞STC‐1和STC‐2的mRNA錶達影響。方法：用化學缺氧劑氯化鈷模擬缺氧環境，用RT‐PCR法分彆檢測在hepG2細胞缺氧和複氧條件下STC‐1、STC‐2的mRNA錶達情況。結果：在hepG2細胞中，STC‐1 mRNA無論在缺氧還是複氧情況下，幾乎不錶達；STC‐2 mRNA在缺氧條件下錶達升高，併隨著缺氧時間延長而升高，複氧後錶達又降低，併隨複氧時間延長而恢複至正常。結論：STC‐2與肝癌的髮生髮展過程密切相關。
목적：연구결양급복양대hepG2세포STC‐1화STC‐2적mRNA표체영향。방법：용화학결양제록화고모의결양배경，용RT‐PCR법분별검측재hepG2세포결양화복양조건하STC‐1、STC‐2적mRNA표체정황。결과：재hepG2세포중，STC‐1 mRNA무론재결양환시복양정황하，궤호불표체；STC‐2 mRNA재결양조건하표체승고，병수착결양시간연장이승고，복양후표체우강저，병수복양시간연장이회복지정상。결론：STC‐2여간암적발생발전과정밀절상관。
Objective To reserch the influence of anoxia‐reoxygen for the expression of STC‐1 and STC‐2 mRNA in hepG2 cell .Methods Using CoCl2 to simulate an anoxia environment ,then detecting the expression of STC‐1 and STC‐2 mRNA of hepG2 cell in different timing during the process of anoxia‐reoxygen .Results There is no expression of STC‐1 mRNA in hepG2 cell during the whole process of anoxia‐reoxygen .But there is an expression of STC‐2 mRNA in hepG2 cell when it is anoxia .The degree of the expression will be increased for the length of the time of anoxia and returned to normal after reoxygen .Conclusion There is a close correlation between STC‐2 and liver cancer in occuring and developing process .
